Processing

Please wait...

Settings

Settings

Goto Application

1. WO1992016840 - LANTHANID FLUORESCENCE ASSAY

Publication Number WO/1992/016840
Publication Date 01.10.1992
International Application No. PCT/FI1992/000072
International Filing Date 13.03.1992
Chapter 2 Demand Filed 07.10.1992
IPC
G01N 33/58 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
CPC
G01N 2458/40
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2458Labels used in chemical analysis of biological material
40Rare earth chelates
G01N 33/582
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
582with fluorescent label
Y10S 436/827
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
436Chemistry: analytical and immunological testing
827Lectins
Applicants
  • OY DATACITY CENTER AB [FI]/[FI]
Inventors
  • XU, Yongyuan
Agents
  • HAKOLA, Unto
Priority Data
91129715.03.1991FI
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) LANTHANID FLUORESCENCE ASSAY
(FR) DOSAGE DE FLUORESCENCE DE LANTHANIDE
Abstract
(EN)
The invention relates to a method based on fluorescence, especially time-resolved flurescence for quantitative assay of a bioaffinity reaction involving bioaffinity components. The method comprises the labelling of one or several of the bioaffinity components participating in the reaction with a lanthanide chelate, forming of a lanthanide chelate for a fluorescence measurement after the reaction, and measuring the fluorescence of the chelate. The lanthanide (Eu, Tb, Sm or Dy) is brought to a strongly fluorescent form before the fluorescence measurement by incorporating the lanthanide in an aggregated particle that comprises the lanthanide chelate and a chelate of a fluorescence-increasing ion (Y, Gd, Tb, Lu or La) to bring about a cofluorescence effect. An aliphatic or aromatic beta-diketone is used as the chelating compound in the aggregate.
(FR)
L'invention se rapporte à un procédé qui se base sur la fluorescence, en particulier la fluorescence à résolution dans le temps, pour effectuer un dosage quantitatif d'une réaction de bioaffinité comprenant des éléments de bioaffinité. Le procédé consiste à marquer un ou plusieurs des éléments de bioaffinité participant à la réaction au moyen d'un chélate de lanthanide, à conférer au chélate de lanthanide une forme propice au mesurage de la fluorescence après la réaction, et à mesurer la fluorescence après la réaction, et à mesurer la fluorescence du chélate. On confère au lanthanide (Eu, Tb, Sm, Dy) une forme fortement fluorescente avant d'effectuer la mesure de fluorescence en incorporant la lanthanide dans une particule agrégée qui se compose du chélate de lanthanide et d'un chélate d'un ion augmentant la fluorescence, afin de produire un effet de cofluorescence. Une bêtadicétone aliphatique ou aromatique est utilisée comme composé de chélation dans l'agrégat.
Also published as
Latest bibliographic data on file with the International Bureau