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1. WO1992016654 - NON-RADIOACTIVE DETERMINATION OF THE PRESENCE OF A GIVEN NUCLEIC ACID IN A BIOLOGICAL SAMPLE

Publication Number WO/1992/016654
Publication Date 01.10.1992
International Application No. PCT/FR1992/000251
International Filing Date 19.03.1992
Chapter 2 Demand Filed 10.10.1992
IPC
C12Q 1/42 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
34involving hydrolase
42involving phosphatase
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G01N 33/84 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
84involving inorganic compounds or pH
CPC
C12Q 1/42
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
42involving phosphatase
C12Q 1/686
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction [PCR]
C12Q 1/703
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
701Specific hybridization probes
702for retroviruses
703Viruses associated with AIDS
G01N 33/84
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
84involving inorganic compounds or pH
Applicants
  • UNIVERSITE DE REIMS CHAMPAGNE-ARDENNE [FR]/[FR] (AllExceptUS)
  • COHEN, Jacques [FR]/[FR] (UsOnly)
  • TABARY, Thierry [FR]/[FR] (UsOnly)
Inventors
  • COHEN, Jacques
  • TABARY, Thierry
Agents
  • GUTMANN, Ernest
Priority Data
91/0339820.03.1991FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) NON-RADIOACTIVE DETERMINATION OF THE PRESENCE OF A GIVEN NUCLEIC ACID IN A BIOLOGICAL SAMPLE
(FR) DETECTION NON RADIOACTIVE DE LA PRESENCE D'UN ACIDE NUCLEIQUE DETERMINE DANS UN ECHANTILLON BIOLOGIQUE
Abstract
(EN)
A method for exposing one or more synthetically-produced nucleotide RNA or DNA sequences in liquid phase and in the presence of excess deoxynucleotides or nucleotides and a predetermined polymerase respectively, from a primer oligonucleotide hybridized with a nucleic acid which is to be detected in a biological sample. Said method includes exposing in the sample the production of pyrophosphate (PPi) resulting from the synthesis reaction of the specific nucleotide sequence(s).
(FR)
L'invention concerne un procédé pour la révélation en phase liquide d'une ou plusieurs séquences nucléotidiques d'ARN ou d'ADN produites par synthèse, en présence respectivement de désoxynucléotides ou de nucléotides en excès et d'une polymérase déterminée, à partir d'un oligonucléotide amorce hybridé avec un acide nucléique que l'on cherche à détecter dans un échantillon biologique, caractérisé en ce qu'il comprend la mise en évidence dans l'échantillon de la production de pyrophosphate (PPi), résultant de la réaction de synthèse de la (ou des) séquence(s) nucléotidique(s) spécifique(s).
Also published as
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