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1. WO1992014742 - METHOD OF SYNTHESIZING SULFURIZED OLIGONUCLEOTIDE ANALOGS

Publication Number WO/1992/014742
Publication Date 03.09.1992
International Application No. PCT/US1991/001008
International Filing Date 14.02.1991
Chapter 2 Demand Filed 27.04.1992
IPC
C07H 21/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
CPC
C07H 21/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Applicants
  • APPLIED BIOSYSTEMS, INC. [US]/[US]
Inventors
  • HIRSCHBEIN, Bernard, L.
Agents
  • SMITH, Joseph, H.
Priority Data
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD OF SYNTHESIZING SULFURIZED OLIGONUCLEOTIDE ANALOGS
(FR) PROCEDE PERMETTANT DE SYNTHETISER DES ANALOGUES D'OLIGONUCLEOTIDES SULFURES
Abstract
(EN)
A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiuram disulfide as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)-containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosphoramidite and/or phosphorthioamidite intermediates.
(FR)
Procédé permettant de synthétiser des analogues d'oligonucléotides sulfurés, tels que des analogues de phosphorothioate ou de phosphorodithioate, dans lequel on utilise un bisulfure de thiuram en tant qu'agent de sulfuration. On peut utiliser le procédé de cette invention pour sulfurer n'importe quel produit intermédiaire renfermant du phosphore (III). On met en pratique ce procédé de préférence dans un synthétiseur d'ADN de type industriel, utilisant des produits intermédiaires de phosphoramidite et/ou de phosphorthioamidite.
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