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1. (WO1992010561) RECOMBINANT CELLS THAT HIGHLY EXPRESS CHROMOSOMALLY-INTEGRATED HETEROLOGOUS GENES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1992/010561 International Application No.: PCT/US1991/008835
Publication Date: 25.06.1992 International Filing Date: 04.12.1991
Chapter 2 Demand Filed: 07.07.1992
IPC:
C12N 9/04 (2006.01) ,C12N 9/88 (2006.01) ,C12N 15/01 (2006.01) ,C12N 15/70 (2006.01) ,C12N 15/74 (2006.01) ,C12N 15/90 (2006.01) ,C12P 7/06 (2006.01)
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
04
acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88
Lyases (4.)
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Recombinant DNA-technology
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Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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Vectors or expression systems specially adapted for E. coli
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
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Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
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Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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Stable introduction of foreign DNA into chromosome
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FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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Preparation of oxygen-containing organic compounds
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containing a hydroxy group
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acyclic
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Ethanol, i.e. non-beverage
Applicants:
UNIVERSITY OF FLORIDA [US/US]; 901-B1 NW 8 Avenue Gainesville, FL 32604, US
Inventors:
INGRAM, Lonnie, O.; US
OHTA, Kazuyoshi; US
Agent:
BENT, Stephen, A. ; Foley & Lardner 3000 K Street, N.W. Suite 500, P.O. Box 25696 Washington, DC 20007-8696, US
Priority Data:
624,22707.12.1990US
Title (EN) RECOMBINANT CELLS THAT HIGHLY EXPRESS CHROMOSOMALLY-INTEGRATED HETEROLOGOUS GENES
(FR) CELLULES RECOMBINEES QUI EXPRIMENT NETTEMENT DES GENES HETEROLOGUES INTEGRES DANS DES CHROMOSOMES
Abstract:
(EN) Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like $i(Zymomonas mobilis), recombinant $i(Escherichia coli) and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
(FR) Selon l'invention, l'on obtient des cellules hôtes recombinées qui comprennent (A) un segment polynucléotidique hétérologue codant pour des polypeptides, intégré de façon stable dans un chromosome, qui est sous le contrôle transcriptionnel d'un promoteur endogène, et (B) une mutation qui effectue l'expression augmentée du segment hétérologue, cela ayant pour résultat une production, par les cellules hôtes de chaque polypeptide codé par ce segment, plus élevée, si l'on compare avec la production de chaque polypeptide, par les cellules hôtes, en l'absence de la mutation. L'expression augmentée ainsi obtenue est retenue en l'absence des conditions favorables aux cellules qui font preuve d'une telle expression augmentée. Lorsque le segment intégré comprend, par exemple, des gènes de la production d'éthanol provenant d'un producteur d'éthanol efficace tel que le $i(Zymomonas mobilis), l'$i(Escherichia coli) recombiné et d'autres cellules bactériennes entériques, selon l'invention, sont capables de transformer en éthanol, de façon efficace, une large gamme de sucres dérivés de la biomasse.
Designated States: AT, AU, BB, BG, BR, CA, CH, CS, DE, DK, ES, FI, GB, HU, JP, KP, KR, LK, LU, MG, MN, MW, NL, NO, PL, RO, SD, SE, SU
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, MC, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
FI932559EP0560885JP2004283176JP3593125JPH06504436CA2097803
AU1991090988DK0560885AU1996061946AU1999018586AU2002035569AU2005248924