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1. (WO1992009698) EXPRESSION OF PACE IN HOST CELLS AND METHODS OF USE THEREOF
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1992/009698 International Application No.: PCT/US1991/008725
Publication Date: 11.06.1992 International Filing Date: 26.11.1991
Chapter 2 Demand Filed: 23.06.1992
IPC:
C07K 14/755 (2006.01) ,C12N 9/64 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
745
Blood coagulation or fibrinolysis factors
755
Factors VIII
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
48
acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50
Proteinases
64
derived from animal tissue, e.g. rennin
Applicants:
GENETICS INSTITUTE, INC. [US/US]; 87 Cambridge Park Drive Cambridge, MA 02140, US
CHIRON CORPORATION [US/US]; 4560 Horton Street Emeryville, CA 94608-2916, US
Inventors:
BARR, Philip, J.; US
BRAKE, Anthony, J.; US
KAUFMAN, Randal, J.; US
TEKAMP-OLSON, Patricia; US
WASLEY, Louise; US
WONG, Polly, A.; US
Agent:
BAK, Mary, E. ; Howson and Howson Spring House Corporate Center P.O. Box 457 Spring House, PA 19002, US
Priority Data:
620,85929.11.1990US
621,09226.11.1990US
621,44329.11.1990US
621,45730.11.1990US
Title (EN) EXPRESSION OF PACE IN HOST CELLS AND METHODS OF USE THEREOF
(FR) EXPRESSION DE PACE DANS DES CELLULES HOTES ET PROCEDES D'UTILISATION
Abstract:
(EN) Compositions and methods are provided for endopeptidase production and for enhanced efficiencies of processing heterologous precursor polypeptides to mature polypeptides, including proteins requiring gamma-carboxylation for biological activity. These compositions and methods utilize recombinant PACE, a mammalian endopeptidase that is specific for dibasic amino acid sites.
(FR) Compositions et procédés de production d'endopeptidase, permettant d'améliorer l'efficacité du traitement de polypeptides précurseurs hétérologues destinés à se transformer en polypeptides matures, comprenant des protéines requérant une gamma-carboxylation en vue d'une activité biologique. Ces compositions et ces procédés utilisent la PACE de recombinaison, une endopeptidase de mammifère qui est spécifique des sites d'acides aminés bibasiques.
Designated States: CA, JP
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0574402JP4236698JPH06504435CA2096418DK0574402