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1. (WO1992009617) CELL STRESS TRANSCRIPTIONAL FACTORS
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What is Claimed is:
1. A DNA fragment encoding a Drosophila heat
shock factor (HSF) protein.
2. A DNA fragment encoding a human heat shock
factor (HSF) protein.
3. The DNA fragment according to claim 1 wherein said fragment has a nucleotide sequence as
defined by Figure 2 (b) or an allelic variation thereof.
4. A DNA fragment encoding a unique portion of
the Drosophila HSF protein defined by Figure
2(b).
5. The DNA fragment according to claim 2 wherein said fragment has a nucleotide sequence as
defined by Figure 12 or allelic variation
thereof.
6. A DNA fragment encoding a unique portion of
the huHSF protein defined by Figure 12.
7. A recombinantly produced Drosophila HSF having the amino acid sequence as defined in Figure
2(b).
8. A recombinantly produced Drosophila HSF
protein having all, or a unique portion of the amino acid sequence given in Figure 2 (b) .
9. A recombinantly produced huHSF protein having the amino acid sequence as defined in Figure
12.
10. A recombinantly produced human HSF protein
encoded by all or a unique portion, of the DNA sequence given in Figure 12.

11. A recombinant DNA molecule comprising
(a) said DNA fragment according to claim 3 or 5, and
(b) a vector, for introducing said DNA
molecule into eukaryotic or prokaryotic host cells.
12. A recombinant DNA molecule according to claim 11 wherein said vector is a plasmid,
bacteriophage or eukaryotic virus vector.
13. The recombinant DNA molecule according to
claim 12 wherein said vector is pJCIO.
14. The recombinant DNA molecule according to
claim 12 wherein said vector is pJCl.
15. A host cell stably transformed or transfected with the recombinant DNA molecule according to claim 11 in a manner allowing expression of said protean encoded in said recombinant DNA molecule.
16. The host cell according to claim 14 wherein said host cell is a prokaryotic cell or a
eukaryotic cell.
17. The host cell according to claim 15 wherein said prokaryotic cell is an Escherichia coli
cell.
18. Purified form antibodies specific for said
protein, or unique fragment thereof, according to claim 8.
19. The antibodies according to claim 18 which are monoclonal.
20. The antibodies according to claim 18 which are polyclonal.

21. The antibodies specific for said HSF protein, or unique fragment thereof, according to claim 10.
22. Purified form antibodies specific for said
protein, or unique fragment thereof, according to claim 10.
23. The antibodies according to claim 21 which are monoclonal.
24. The antibodies according to claim 21 which are polyclonal.
25. A method for the detection of human HSF
protein in a sample comprising the steps of:
(i) contacting a reagent which specifically
reacts with said protein with said sample under conditions such that a reaction
between said reagent and said protein can be effected, and
(ii) detecting the presence or absence of said reaction.
26. The method according to claim 25 wherein said reagent is an antibody.
27. A method of diagnosing abnormal stress
conditions, including disease, comprising the steps of:
(i) contacting a biological sample with
antibody according to claim 21 under
conditions wherein the reaction comprising the binding of said antibody to said protein is effected and
(ii) detecting the presence or absence of said reaction in the nucleus of said cellular
sample.

28. A method of diagnosing stress conditions in human cells by measuring the presence
multimeric state of human HSF (HuHSF)
comprising the steps of:
(i) separating crude or fractionated human
cell extracts by nondenaturing gel
electrophoresis,
(ii) transferring gel to solid support,
(iii) reacting anti-HuHSF antibodies to solid support under conditions such that anti- HuHSF complexes are formed, and
(iv) detecting the presence of multimeric
complexes.
29. The method of diagnosing stress conditions in human cells by measuring the presence of
multimeric human HSF (HuHSF) comprising the steps of:
(i) reacting crude or fractionated human cell extracts with a chemical cross-linkng
reagent, wherein said reagent is not
glutaraldehyde,
(ii) separating chemically cross-linked crude and fractionated human extracts by
denaturing gel electrophoresis,
(iϋ) transforming gel to solid support,
(iv) reacting said support with anti-HuHSF
antibodies under conditions such that
antibody-HSF complexes are formed, and
(v) detecting the presence multimeric HSF
complexes.

30. A method for constructing chimeric proteins which are subject to multimerization in a
stress regulated manner comprising the steps of:
(i) linking a gene encoding a protein or
peptide to a metazoan HSF gene fragment encoding HSF DNA protein in frame, to
produce a recombinant chimeric molecule, (ii) expressing said recombinant DNA molecule in a host cell stably transformed or
transfected with said molecule, and
(iii) stressing said chimeric proteins
expressed in said host cell under conditions such that multimeric conplexes of chimeric proteins are formed.
31. A method for measuring chronic or abnormal stress conditions in a tissue comprising the steps of:
(i) linking a metazoan HSF gene fragment
encoding HSF protein to a tissue-general or tissue-specific promoter to produce a recombinant molecule,
(ii) transfecting said recombinant molecule
into a transgenic mouse,
(iϋ) adding a stress inducer to said
transgenic mouse, and
(iv) measuring the absence or presence of
multimerization of said HSF protein to
determine the presence of the stress
condition.