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1. (WO1992001047) METHODS FOR PRODUCING MEMBERS OF SPECIFIC BINDING PAIRS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1992/001047 International Application No.: PCT/GB1991/001134
Publication Date: 23.01.1992 International Filing Date: 10.07.1991
Chapter 2 Demand Filed: 27.01.1992
IPC:
C07K 16/00 (2006.01) ,C07K 16/18 (2006.01) ,C07K 16/34 (2006.01) ,C07K 16/40 (2006.01) ,C07K 16/44 (2006.01) ,C07K 16/46 (2006.01) ,C07K 19/00 (2006.01) ,C12N 15/10 (2006.01) ,C12N 15/62 (2006.01) ,C12N 15/73 (2006.01) ,G01N 33/53 (2006.01) ,G01N 33/68 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18
against material from animals or humans
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18
against material from animals or humans
34
against blood group antigens
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
40
against enzymes
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
44
against material not provided for elsewhere
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
46
Hybrid immunoglobulins
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
19
Hybrid peptides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
62
DNA sequences coding for fusion proteins
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70
Vectors or expression systems specially adapted for E. coli
73
Expression systems using phage lambda regulatory sequences
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68
involving proteins, peptides or amino acids
Applicants:
CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED [GB/GB]; The Daly Research Laboratories Babraham Hall Cambridge Cambridgeshire CB2 4AT, GB (AllExceptUS)
MEDICAL RESEARCH COUNCIL [GB/GB]; 20 Park Crescent London W1N 4AL, GB (AllExceptUS)
McCAFFERTY, John [GB/GB]; GB (UsOnly)
POPE, Anthony, Richard [GB/GB]; GB (UsOnly)
JOHNSON, Kevin, Stuart [GB/GB]; GB (UsOnly)
HOOGENBOOM, Hendricus, Renerus, Jacobus, Mattheus [NL/GB]; GB (UsOnly)
GRIFFITHS, Andrew, David [GB/GB]; GB (UsOnly)
JACKSON, Ronald, Henry [GB/GB]; GB (UsOnly)
HOLLIGER, Kaspar, Philipp [CH/GB]; GB (UsOnly)
MARKS, James, David [US/GB]; GB (UsOnly)
CLACKSON, Timothy, Piers [GB/GB]; GB (UsOnly)
CHISWELL, David, John [GB/GB]; GB (UsOnly)
WINTER, Gregory, Paul [GB/GB]; GB (UsOnly)
BONNERT, Timothy, Peter [GB/GB]; GB (UsOnly)
Inventors:
McCAFFERTY, John; GB
POPE, Anthony, Richard; GB
JOHNSON, Kevin, Stuart; GB
HOOGENBOOM, Hendricus, Renerus, Jacobus, Mattheus; GB
GRIFFITHS, Andrew, David; GB
JACKSON, Ronald, Henry; GB
HOLLIGER, Kaspar, Philipp; GB
MARKS, James, David; GB
CLACKSON, Timothy, Piers; GB
CHISWELL, David, John; GB
WINTER, Gregory, Paul; GB
BONNERT, Timothy, Peter; GB
Agent:
MEWBURN, Ellis; 2 Cursitor Street London EC4A 1BQ, GB
Priority Data:
9015198.610.07.1990GB
9022845.319.10.1990GB
9024503.612.11.1990GB
9104744.906.03.1991GB
9110549.415.05.1991GB
Title (EN) METHODS FOR PRODUCING MEMBERS OF SPECIFIC BINDING PAIRS
(FR) PROCEDE DE PRODUCTION DE CHAINON DE PAIRES A LIAISON SPECIFIQUE
Abstract:
(EN) A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
(FR) On identifie un membre d'une paire à liaison spécifique (sbp) en exprimant l'ADN codant une colonie à diversité génétique de ces membres de sbp dans des cellules hôtes recombinées dans lesquelles les membres de sbp se présentent sous forme fonctionnelle à la surface d'un capside de présentation génétique de recombinaison secrété (rgdp) renfermant l'ADN codant le membre sbp ou un constituant polypeptidique de celui-ci, grâce à l'expression de ce membre de sbp ou de son constituant polypeptidique sous forme d'une fusion avec un constituant d'encapsidation du rgdp. On peut sélectionner les sbp présentées selon l'affinité avec un membre de sbp complémentaire, et récupérer l'ADN des rgdp sélectionnés pour l'expression des membres de sbp sélectionnés. On peut ainsi obtenir des membres anticorporaux de sbp dont les diverses chaînes sont exprimées, l'une étant fusionnée au constituant d'encapsidation et l'autre étant sous forme libre permettant une association au polypeptide partenaire de fusion. Un phagemide peut servir de vecteur d'expression et la fusion d'encapsidation contribue à l'encapsidation de l'ADN du phagemide. A l'aide de ce procédé, on peut associer des banques d'ADN codant des chaînes respectives de ces membres multimères de sbp, afin d'obtenir des membres de sbp ayant une plus grande diversité génétique que celle que l'on obtiendrait facilement à l'aide des procédés traditionnels.
Designated States: AT, AU, BB, BG, BR, CA, CH, DE, DK, ES, FI, GB, HU, JP, KP, KR, LK, LU, MC, MG, MN, MW, NL, NO, PL, RO, SD, SE, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)