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1. (WO1991018117) REPLICATABLE HYBRIDIZABLE RECOMBINANT RNA PROBES AND HYBRIDIZATION ASSAY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/018117 International Application No.: PCT/US1991/003634
Publication Date: 28.11.1991 International Filing Date: 23.05.1991
Chapter 2 Demand Filed: 23.12.1991
IPC:
C12Q 1/68 (2006.01) ,C12Q 1/70 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
70
involving virus or bacteriophage
Applicants:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK [US/US]; Broadway & West 116th Street New York, NY 10012, US
Inventors:
KRAMER, Fred, R.; US
LIZARDI, Paul, M.; MX
Agent:
WHITE, John, P.; Cooper & Dunham 30 Rockefeller Plaza New York, NY 10112, US
Priority Data:
527,58523.05.1990US
Title (EN) REPLICATABLE HYBRIDIZABLE RECOMBINANT RNA PROBES AND HYBRIDIZATION ASSAY
(FR) SONDES D'ARN RECOMBINE HYBRIDABLE REPLICABLE ET TEST D'HYBRIDATION
Abstract:
(EN) The present invention provides a replicatable and hybridizable recombinant single-stranded RNA probe molecule comprising: a recognition sequence for the binding of an RNA-directed RNA polymerase; a sequence required for the initiation of product strand synthesis by the polymerase; and a heterologous RNA sequence inserted at a specific site in the internal region of the recombinant molecule and complementary to an oligo- or polynucleotide of interest. This invention also provides methods for determining the presence of concentration of an oligo- or polynucleotide of interest in a sample and for simultaneously determining the presence or concentration of several different oligo- or polynucleotides of interest in a sample.
(FR) Une molécule de sonde d'ARN monocaténaire recombiné replicable et hybridable comprend une séquence de reconnaissance permettant de lier une polymérase d'ARN à correspondance ARN; une séquence requise pour lancer la synthèse de brins du produit au moyen de la polymérase; et une séquence d'ARN hétérologue insérée dans un site spécifique dans la région interne de la molécule recombinée, complémentaire d'un oligonucléotide ou d'un polynucléotide à étudier. Des procédés permettent de déterminer la présence d'une concentration d'un oligonucléotide ou d'un polynucléotide à étudier dans un échantillon et en même temps de déterminer la présence ou la concentration de plusieurs oligonucléotides ou polynucléotides différents à étudier dans un échantillon.
Designated States: AU, CA, JP
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AU1991079977