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1. (WO1991018114) POLYNUCLEOTIDE AMPLIFICATION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/018114 International Application No.: PCT/GB1991/000803
Publication Date: 28.11.1991 International Filing Date: 22.05.1991
Chapter 2 Demand Filed: 20.12.1991
IPC:
C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
MEDICAL RESEARCH COUNCIL [GB/GB]; 20 Park Crescent London W1, GB (AllExceptUS)
JONES, David, Stephen, Charnock [GB/GB]; GB (UsOnly)
ROSENTHAL, Andre [GB/GB]; GB (UsOnly)
Inventors:
JONES, David, Stephen, Charnock; GB
ROSENTHAL, Andre; GB
Agent:
MERCER, Christopher, Paul ; Carpmaels & Ransford 43 Bloomsbury Square London WC1A 2RA, GB
Priority Data:
9011454.722.05.1990GB
Title (EN) POLYNUCLEOTIDE AMPLIFICATION
(FR) AMPLIFICATION DE POLYNUCLEOTIDES
Abstract:
(EN) The polynucleotide amplification method described includes the use of a labelled primer that is complementary to a specific known sequence in a target strand. A linear polymerase chain reaction (PCR) step is first conducted with the labelled primer. The labelled linear extension products are then isolated by means of a suitable support matrix that cooperatively binds to the label. The labelled extension products can then be subjected to exponential PCR in the absence of any other strands.
(FR) La méthode d'amplification de polynucléotides décrite consiste à utiliser une amorce marquée complémentaire à une séquence spécifique connue dans un brin cible. Une étape de réaction en chaîne de polymérase (RCP) linéaire est tout d'abord effectuée avec l'amorce marquée. Les produits d'extension linéaire marqués sont ensuite isolés par l'intermédiare d'une matrice de support appropriée qui se lie de manière coopérante à l'étiquette de marquage. Les produits d'extension marqués peuvent alors être soumis à la RCP exponentielle en l'absence de tout autre brin.
Designated States: JP, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0530243JPH05508313