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1. (WO1991018109) TEMPERATURE CHANGE INDICATOR EMPLOYING ENZYMES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/018109 International Application No.: PCT/GB1991/000815
Publication Date: 28.11.1991 International Filing Date: 23.05.1991
Chapter 2 Demand Filed: 12.12.1991
IPC:
C12Q 1/00 (2006.01) ,C12Q 1/26 (2006.01) ,G01N 31/22 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
26
involving oxidoreductase
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
31
Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroups; Apparatus specially adapted for such methods
22
using chemical indicators
Applicants:
RAYBOURNE LIMITED [GB/GB]; 124 Willoughby House The Barbican London EC2Y 1BL, GB (AllExceptUS)
KOZLOV, Igor, Anatolievich [SU/GB]; GB (UsOnly)
PARTON, Adrian [GB/GB]; GB (UsOnly)
Inventors:
KOZLOV, Igor, Anatolievich; GB
PARTON, Adrian; GB
Agent:
RAYNOR, John; W.H. Beck, Greener & Co. 7 Stone Buildings Lincoln's Inn London WC2A 3SZ, GB
Priority Data:
9011677.324.05.1990GB
Title (EN) TEMPERATURE CHANGE INDICATOR EMPLOYING ENZYMES
(FR) INDICATEUR DE CHANGEMENT DE TEMPERATURE UTILISANT DES ENZYMES
Abstract:
(EN) Enzyme reactions may be carried out in a non-aqueous medium comprising a C12-C20 aliphatic alcohol such as lauryl alcohol. The medium may be a mixture of such an alcohol with a C6-C30 aliphatic hydrocarbon such as hexane or decane. The principle may be used to produce a temperature change indicator, comprising a solid non-aqueous medium comprising a C12-C20 aliphatic alcohol such as lauryl alcohol and a C6-C30 aliphatic hydrocarbon such as hexane or decane, together with an enzyme, a substrate for the enzyme, and a development reagent capable of producing a detectable change, on reaction of the substrate with the enzyme. The medium is chosen to melt at a specific temperature, or in a specific temperature range, to permit reaction to occur between the enzyme, the substrate, and the development reagent. Preferably, the enzyme is mushroom phenol oxidase, the enzyme substrate is $i(o)-dihydroxyphenol, and the development reagent is an ester of an amino acid with a C4-C20 alcohol, for example the butyl ester of proline.
(FR) Des réactions enzymatiques peuvent être effectuées dans un milieu non aqueux comprenant un alcool aliphatique C12-C20 tel que l'alcool laurique. Le milieu peut être composé d'un mélange d'un tel alcool avec un hydrocarbure aliphatique C6-C30 tel que l'hexane ou le décane. Le principe peut être utilisé pour produire un indicateur de changement de température, comprenant un milieu solide non aqueux composé d'un alcool aliphatique C12-C20 tel que l'alcool laurique et un hydrocarbure aliphatique C6-C30 tel que l'hexane ou le décane, avec un enzyme, un substrat pour l'enzyme et un réactif de développement capable de produire un changement détectable lorsque le substrat réagit avec l'enzyme. Le milieu est choisi de manière à fondre à une température spécifique ou dans une plage spécifique de température, afin qu'une réaction ait lieu entre l'enzyme, le substrat et le réactif de développement. De préférence, l'enzyme est de l'oxydase de phénol de champignon, le substrat d'enzyme est du $i(o)-dihydroxyphénol, et le réactif de développement est un ester d'un acide aminé avec un alcool C4-C20, par example l'ester butylique de proline.
Designated States: AU, BR, CA, GB, HU, JP, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0532554AU1991079025