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1. (WO1991018103) METHOD AND DEVICE FOR MAKING LIVING CELLS PERMEABLE
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/018103 International Application No.: PCT/BE1991/000030
Publication Date: 28.11.1991 International Filing Date: 16.05.1991
Chapter 2 Demand Filed: 09.12.1991
IPC:
A01H 1/06 (2006.01) ,C12M 3/00 (2006.01) ,C12N 13/00 (2006.01) ,C12N 15/87 (2006.01)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1
Processes for modifying genotypes
06
Processes for producing mutations, e.g. treatment with chemicals or with radiation
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3
Tissue, human, animal or plant cell, or virus culture apparatus
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
13
Treatment of micro-organisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Applicants:
SCIENTIFIC EQUIPMENT DESIGN & DEVELOPMENT S.C. [BE/BE]; 31, rue de la Casmaterie B-4051 Chaudfontaine, BE (AllExceptUS)
SERVAIS, Christian [BE/BE]; BE (UsOnly)
LOUETTE, Joël [BE/BE]; BE (UsOnly)
Inventors:
SERVAIS, Christian; BE
LOUETTE, Joël; BE
Agent:
VAN MALDEREN, Michel; Offive Van Malderen 85/043, boulevard de la Sauvenière B-4000 Liège, BE
Priority Data:
900051816.05.1990BE
Title (EN) METHOD AND DEVICE FOR MAKING LIVING CELLS PERMEABLE
(FR) PROCEDE ET DISPOSITIF DE PERMEABILISATION DE CELLULES VIVANTES
Abstract:
(EN) A method and a device for making cell membranes permeable using electrical discharges (electroporation), wherein at least one short electrical discharge is applied to a suspension containing cells to be made permeable and molecules to be accommodated therein, in order to generate in said suspension a high-value electric field, whereafter at least one longer electrical discharge is applied thereto to generate a lower-value electric field therein.
(FR) L'invention concerne un procédé et dispositif de perméabilisation de membranes cellulaires par décharge électrique (électroporation) dans lequel on applique à une suspension contenant des cellules à rendre perméables, ainsi que des molécules destinées à être logées à l'intérieur des cellules, successivement, au moins une décharge électrique de courte durée engendrant dans la suspension un champ électrique de valeur élevée et ensuite au moins une décharge électrique de plus longue durée et engendrant dans la suspension un champ électrique de valeur moins élevée.
Designated States: AU, BB, BG, BR, CA, FI, HU, JP, KP, KR, LK, MC, MG, MW, NO, PL, RO, SD, SU, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, ML, MR, SN, TD, TG)
Publication Language: French (FR)
Filing Language: French (FR)
Also published as:
AU1991077722