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1. (WO1991018102) SHUTTLE VECTOR FOR RECOMBINANT $i(BACILLUS THURINGIENSIS) STRAIN DEVELOPMENT
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/018102 International Application No.: PCT/US1991/003360
Publication Date: 28.11.1991 International Filing Date: 14.05.1991
Chapter 2 Demand Filed: 23.11.1991
IPC:
C07K 14/325 (2006.01) ,C12N 15/75 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
32
from Bacillus (G)
325
Bacillus thuringiensis crystal peptide (delta-endotoxin)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
75
for Bacillus
Applicants:
ECOGEN INC. [US/US]; 2005 Cabot Boulevard West Langhorne, PA 19047-1810, US
Inventors:
BAUM, James, A.; US
Agent:
NADEL, Alan, S.; Panitch, Schwarze, Jacobs & Nadel 1601 Market Street, 36th Floor Philadelphia, PA 19103 , US
Priority Data:
523,67115.05.1990US
Title (EN) SHUTTLE VECTOR FOR RECOMBINANT $i(BACILLUS THURINGIENSIS) STRAIN DEVELOPMENT
(FR) VECTEUR NAVETTE SERVANT A LA CULTURE DE SOUCHES DE $i(BACILLUS THURINGIENSIS) DE RECOMBINAISON
Abstract:
(EN) A plasmid shuttle vector, bifunctional in $i(Bacillus thuringiensis) $i(Bt) and $i(E.coli) hosts, containing a $i(Bt)-derived origin of replication, an origin of replication functional in $i(E.coli), a multiple cloning site, and optionally antibiotic resistance marker genes. The elements of the plasmid shuttle vector are located on removable DNA fragments, making the vector useful for the development of recombinant $i(Bt) strains that contain not only one or more insecticidal $i(Bt) toxin genes but are also essentially free of DNA from non-$i(Bacillus) biological sources.
(FR) Vecteur navette plasmide bifonctionnel dans des hôtes de $i(Bacillus thuringiensis) $i(Bt) et $i(E. coli) contenant une origine de réplication dérivée de $i(Bt), une origine de réplication fonctionnelle dans $i(E.coli), un site de clonage multiple, et éventuellement des gènes marqueurs de résistance aux antibiotiques. Les éléments du vecteur navette plasmide se trouvent sur des fragments d'ADN amovibles, ce qui rend le vecteur utile pour la culture de souches de $i(Bt) de recombinaison qui ne contiennent pas seulement un ou plusieurs gènes de toxine $i(Bt) insecticides mais qui sont aussi essentiellement exemptes d'ADN de sources biologiques non-$i(Bacillus).
Designated States: AU, BR, CA, FI, HU, JP, KR, NO, SU
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0533701JP2916255JPH05507408CA2082821AU1991079847