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1. (WO1991017427) METHOD FOR DETERMINING A SPECIFIC LIGAND IN A FLUID SAMPLE WITH THE AID OF AN EVANESCENT FIELD, AND ALSO A COMPONENT OF THE REQUISITE MEASURING EQUIPMENT SUITABLE FOR THIS PURPOSE
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/017427 International Application No.: PCT/NL1991/000073
Publication Date: 14.11.1991 International Filing Date: 01.05.1991
Chapter 2 Demand Filed: 26.11.1991
IPC:
G01N 21/55 (2006.01) ,G01N 33/543 (2006.01) ,G01N 33/553 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
17
Systems in which incident light is modified in accordance with the properties of the material investigated
55
Specular reflectivity
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
551
the carrier being inorganic
553
Metal or metal coated
Applicants:
NEDERLANDSE ORGANISATIE VOOR TOEGEPAST-NATUURWETENSCHAPPELIJK ONDERZOEK TNO [NL/NL]; Schoemakersstraat 97 NL-2628 VK Delft, NL (AllExceptUS)
SCHASFOORT, Richardus, Bernardus, Maria [NL/NL]; NL (UsOnly)
Inventors:
SCHASFOORT, Richardus, Bernardus, Maria; NL
Agent:
DE BRUIJN, Leendert, C.; Nederlandsch Octrooibureau Scheveningseweg 82 P.O. Box 29720 NL-2502 LS The Hague, NL
Priority Data:
900105202.05.1990NL
Title (EN) METHOD FOR DETERMINING A SPECIFIC LIGAND IN A FLUID SAMPLE WITH THE AID OF AN EVANESCENT FIELD, AND ALSO A COMPONENT OF THE REQUISITE MEASURING EQUIPMENT SUITABLE FOR THIS PURPOSE
(FR) PROCEDE DE DETERMINATION D'UN LIGAND SPECIFIQUE DANS UN ECHANTILLON FLUIDE A L'AIDE D'UN CHAMP EVANESCENT, ET COMPOSANT DU MATERIEL DE MESURE NECESSAIRE A CETTE FIN
Abstract:
(EN) The invention relates to a method for determining a ligand in a fluid sample by bringing the sample into contact with a surface of an optical element which is capable of generating an evanescent field and which surface is provided with a specific binding partner for the ligand to be determined; irradiating the optical element with light of a wavelength suitable for production of the evanescent field; and analysing the reflected light in order to determine the possibly modified evanescent field characteristics of the optical element as a consequence of the formation of a complex between the ligand and the specific binding partner in which method the characteristic feature employed is a multiplicity of supports in particle form which carry the ligand or binding partners specific for the ligand.
(FR) Procédé de détermination d'un ligand dans un échantillon fluide consistant à mettre l'échantillon en contact avec la surface d'un élément optique apte à générer un champ évanescent, ladite surface étant dotée d'un partenaire de liaison spécifique pour le ligand à déterminer; à irradier l'élément optique avec de la lumière dont la longueur d'onde est adaptée pour la production du champ évanescent; et à analyser la lumière réfléchie afin de déterminer toutes modifications des caractéristiques du champ évanescent de l'élément optique qui seraient dues à la formation d'un complexe entre le ligand et le partenaire de liaison spécifique. Le procédé est caractérisé par l'utilisation d'une pluralité de supports sous forme particulaire qui portent le ligand ou des partenaires de liaison spécifiques pour celui-ci.
Designated States: AT, AU, BB, BG, BR, CA, CH, DE, DK, ES, FI, GB, HU, JP, KP, KR, LK, LU, MC, MG, MW, NL, NO, PL, RO, SD, SE, SU, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, ML, MR, SN, TD, TG)
Publication Language: English (EN)
Filing Language: Dutch (NL)
Also published as:
EP0600862JPH06502717AU1991078714