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1. (WO1991017270) METHOD FOR REDUCING CARRYOVER CONTAMINATION IN AN AMPLIFICATION PROCEDURE
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/017270 International Application No.: PCT/US1991/003052
Publication Date: 14.11.1991 International Filing Date: 30.04.1991
IPC:
C12Q 1/68 (2006.01) ,C12Q 1/70 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
70
involving virus or bacteriophage
Applicants:
AMGEN INC. [US/US]; 1840 Dehavilland Drive Thousand Oaks, CA 91320-1789, US
Inventors:
RICHARDS, Rodney, M.; US
JONES, Theodore; US
SNITMAN, David, L.; US
BROWN, Gregory, S.; US
Agent:
ODRE, Steven, M. ; Amgen Inc. 1840 Dehavilland Drive Thousand Oaks, CA 91320-1789, US
Priority Data:
517,63101.05.1990US
686,47819.04.1991US
Title (EN) METHOD FOR REDUCING CARRYOVER CONTAMINATION IN AN AMPLIFICATION PROCEDURE
(FR) PROCEDE DE REDUCTION DE LA CONTAMINATION ENTRAINEE DANS UN PROCESSUS D'AMPLIFICATION
Abstract:
(EN) The present invention provides an efficient and economical method for reducing carryover contamination in an amplification procedure. The method of the present invention enables background caused by contaminant amplification product to be reduced or eliminated through the incorporation of at least one modification into the amplification product. The modified amplification product is readily distinguishable from the target sequence in a test sample. Prior to amplifying the target in a new test sample, the sample may be treated to selectively eliminate the contaminant amplification product so that it cannot be amplified in the new sample.
(FR) L'invention se rapporte à un procédé efficace et économique de réduction de la contamination entraînée dans un processsus d'amplification. Ce procédé permet de réduire ou d'éliminer les effets de fond causés par le produit d'amplification contaminant par l'intermédiaire de l'incorporation d'au moins une modification dans le produit d'amplification. Le produit d'amplification ainsi modifié peut être facilement distingué de la séquence cible dans un échantillon test. Avant d'amplifier la cible dans un nouvel échantillon test, on peut traiter cet échantillon afin d'éliminer sélectivement le produit d'amplification contaminant, de façon à ce qu'il ne puisse pas être amplifié dans le nouvel échantillon.
Designated States: AU, BR, CA, FI, HU, JP, KR, NO, SU
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
FI916171NO19915136EP0481069CA2063432KR1019920702726AU1991079871