Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO1991017264) ASSAY FOR SPECIFIC NUCLEIC ACID SEQUENCES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/017264 International Application No.: PCT/GB1991/000696
Publication Date: 14.11.1991 International Filing Date: 01.05.1991
Chapter 2 Demand Filed: 11.11.1991
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
IMPERIAL CHEMICAL INDUSTRIES PLC [GB/GB]; Imperial Chemical House Millbank London SW1P 3JF, GB (AllExceptUS)
COPLEY, Clive, Graham [GB/GB]; GB (UsOnly)
BOOT, Christopher [GB/GB]; GB (UsOnly)
Inventors:
COPLEY, Clive, Graham; GB
BOOT, Christopher; GB
Agent:
LOCKE, Timothy, John; Imperial Chemical Industries plc P.O. Box 6 Bessemer Road Welwyn Garden City Hertfordshire AL7 1HD, GB
Priority Data:
9009903.702.05.1990GB
Title (EN) ASSAY FOR SPECIFIC NUCLEIC ACID SEQUENCES
(FR) ANALYSE DE SEQUENCES SPECIFIQUES D'ACIDE NUCLEIQUE
Abstract:
(EN) An assay method in which a target nucleic acid sequence is detected using a labelled probe comprising a complementary nucleic acid sequence under conditions where the target and probe hybridise and a nuclease requiring double stranded DNA for activity degrades the probe faster than the target. A cycling effect leads to large amount of degraded probe being formed, relative to the amount of target, providing an amplification of detectable degradation products. Also described are new oligonucleotides and kits.
(FR) Un procédé d'analyse dans lequel on détecte une séquence cible d'acide nucléique en utilisant une sonde marquée comprenant une séquence complémentaire d'acide nucléique dans des conditions causant l'hybridation de la cible et de la sonde et où une nucléase nécessitant un ADN à double brin pour son activité dégrade la sonde plus rapidement que la cible. Un effet cyclique conduit à la formation de quantités importantes de sonde dégradée, par rapport à l'importance de la cible, ce qui implique une amplification des produits de dégradation détectables. L'invention décrit également de nouveaux oligonucléotides et des 'kits' d'analyse.
Designated States: JP, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)