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1. (WO1991017251) STEROID 5$g(a)-REDUCTASES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/017251 International Application No.: PCT/US1991/002866
Publication Date: 14.11.1991 International Filing Date: 25.04.1991
Chapter 2 Demand Filed: 28.11.1991
IPC:
C12N 9/02 (2006.01) ,C12Q 1/26 (2006.01) ,C12Q 1/32 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
26
involving oxidoreductase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
26
involving oxidoreductase
32
involving dehydrogenase
Applicants:
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM [US/US]; 201 West 7th Street Austin, TX 78701, US
Inventors:
ANDERSON, Stefan; US
RUSSELL, David, W.; US
Agent:
PARKER, David, L.; Arnold, White & Durkee P.O. Box 443 Houston, TX 77210, US
GRUNECKER, KINKELDEY, STOCKMAIR & PARTNER; Maximillianstrasse 58 D-8000 Munich 22, DE
Priority Data:
517,66130.04.1990US
Title (EN) STEROID 5$g(a)-REDUCTASES
(FR) 5$g(a)-REDUCTASES STEROIDIENNES
Abstract:
(EN) Disclosed are methods and compositions for the preparation of human steroid 5$g(a)-reductases by recombinant means, as well as for the use of these enzymes in screening assays for the identification of compounds which have the ability to inhibit or otherwise alter the enzymatic function of these enzymes. In particular embodiments, methods and compositions are disclosed for the preparation of steroid 5$g(a)-reductases from human sources, as well as DNA sequences which encode these enzymes. These sequences may be used directly in the preparation of genetic constructs, or may be employed in the preparation of hybridization probes for the selection of enzyme-encoding sequences from other sources.
(FR) L'invention décrit des procédés et des compositions servant à préparer des 5$g(a)-réductases stéroïdiennes humaines par des moyens recombinants, ainsi que l'utilisation de ces enzymes dans des dosages de criblage en vue de l'identification de composés ayant la capacité d'inhiber ou de modifier d'une autre manière la fonction enzymatique de ces enzymes. Dans des modes de réalisation préférés, la présente invention décrit des procédés et des compositions servant à préparer des 5$g(a)-réductases stéroïdiennes à partir de sources humaines, ainsi que des séquences d'ADN qui codent pour ces enzymes. Ces séquences peuvent être utilisées directement dans la préparation de structures génétiques ou elles peuvent être employées dans la préparation de sondes d'hybridation en vue de la sélection de séquences de codage d'enzymes provenant d'autres sources.
Designated States: AT, AU, BB, BG, BR, CA, CH, DE, DK, ES, FI, GB, HU, JP, KP, KR, LK, LU, MC, MG, MW, NL, NO, PL, RO, SD, SE, SU
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CM, GA, ML, MR, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0528906JPH05507405CA2079454AU1991078579