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1. (WO1991017247) PROTEASE DEFICIENT BACTERIAL HOSTS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/017247 International Application No.: PCT/US1991/003012
Publication Date: 14.11.1991 International Filing Date: 02.05.1991
Chapter 2 Demand Filed: 05.11.1991
IPC:
C07K 14/195 (2006.01) ,C12N 9/06 (2006.01) ,C12N 9/52 (2006.01) ,C12N 15/70 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
06
acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
48
acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50
Proteinases
52
derived from bacteria
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70
Vectors or expression systems specially adapted for E. coli
Applicants:
THE GENERAL HOSPITAL CORPORATION [US/US]; Fruit Street Boston, MA 02114, US
Inventors:
HUALA, Eva; US
AUSUBEL, Frederick, M.; US
Agent:
GOLDSTEIN, Jorge, A. ; Sterne, Kessler, Goldstein & Fox 1225 Connecticut Avenue, N.W. Suite 300 Washington, DC 20036, US
Priority Data:
515,94002.05.1990US
Title (EN) PROTEASE DEFICIENT BACTERIAL HOSTS
(FR) HOTES BACTERIENS A CARENCE EN PROTEASE
Abstract:
(EN) $i(E. coli) mutants which are capable of expressing cloned proteins in a highly stable manner are described.
(FR) Mutants de $i(E. coli) capables d'exprimer des protéines clonées de manière hautement stable.
Designated States: AU, CA, HU, JP, KR, SU
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0528955JPH05506996CA2081342AU1991078990DK0528955