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1. (WO1991017239) A THERMOSTABLE LIGASE MEDIATED DNA AMPLIFICATION SYSTEM FOR THE DETECTION OF GENETIC DISEASES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/017239 International Application No.: PCT/US1991/002968
Publication Date: 14.11.1991 International Filing Date: 29.04.1991
Chapter 2 Demand Filed: 30.10.1991
IPC:
C12N 9/00 (2006.01) ,C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
CORNELL RESEARCH FOUNDATION, INC. [US/US]; 20 Thornwood Drive Ithaca, NY 14850, US (AllExceptUS)
CALIFORNIA INSTITUTE OF TECHNOLOGY [US/US]; 1301 E. California Boulevard Pasadena, CA 91125, US (AllExceptUS)
BARANY, Francis [US/US]; US (UsOnly)
ZEBALA, John [US/US]; US (UsOnly)
NICKERSON, Deborah, A. [US/US]; US (UsOnly)
KAISER, Robert, J., Jr. [US/US]; US (UsOnly)
HOOD, Leroy, E. [US/US]; US (UsOnly)
Inventors:
BARANY, Francis; US
ZEBALA, John; US
NICKERSON, Deborah, A.; US
KAISER, Robert, J., Jr.; US
HOOD, Leroy, E.; US
Agent:
YAHWAK, George, M.; Yahwak & Associates 25 Skytop Drive Trumbull, CT 06611, US
Priority Data:
518,44703.05.1990US
Title (EN) A THERMOSTABLE LIGASE MEDIATED DNA AMPLIFICATION SYSTEM FOR THE DETECTION OF GENETIC DISEASES
(FR) SYSTEME D'AMPLIFICATION D'ADN INDUIT PAR LIGASE THERMOSTABLE, SERVANT A DETECTER DES MALADIES GENETIQUES.
Abstract:
(EN) The present invention relates to the cloning of the gene of a thermophilic DNA ligase, from $i(Thermus aquaticus) strain HB8, and the use of this ligase for the detection of specific sequences of nucleotides in a variety of nucleic acid samples, and more particularly in those samples containing a DNA sequence characterized by a difference in the nucleic acid sequence from a standard sequence including single nucleic acid base pair changes, deletions, insertions or translocations.
(FR) Cette invention concerne le clonage du gène d'une ligase d'ADN thermophile, provenant de la souche $i(Thermus aquaticus) HB8, et l'utilisation de cette ligase pour détecter des séquences spécifiques de nucléotides dans plusieurs échantillons d'acides nucléiques et plus particulièrement dans les échantillons renfermant une séquence d'ADN qui se caractérise par une différence entre la séquence d'acide nucléique et une séquence standard comprenant des changements, des suppressions, des insertions ou des transferts de paires de base individuelle d'acide nucléique.
Designated States: JP, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0528882JPH05508764DK0528882