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1. (WO1991017169) PHOSPHORAMIDITE DERIVATIVES, THEIR PREPARATION AND THE USE THEREOF IN THE INCORPORATION OF REPORTER GROUPS ON SYNTHETIC OLIGONUCLEOTIDES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/017169 International Application No.: PCT/GB1991/000713
Publication Date: 14.11.1991 International Filing Date: 03.05.1991
Chapter 2 Demand Filed: 30.10.1991
IPC:
C07F 9/24 (2006.01) ,C07F 9/6561 (2006.01) ,C07H 21/00 (2006.01) ,C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
F
ACYCLIC, CARBOCYCLIC, OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
9
Compounds containing elements of the 5th Group of the Periodic System
02
Phosphorus compounds
06
without P-C bonds
22
Amides of acids of phosphorus
24
Esteramides
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
F
ACYCLIC, CARBOCYCLIC, OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
9
Compounds containing elements of the 5th Group of the Periodic System
02
Phosphorus compounds
547
Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
6561
containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
AMERSHAM INTERNATIONAL PLC [GB/GB]; Amersham Place Little Chalfont Buckinghamshire HP7 9NA, GB (AllExceptUS)
MISIURA, Konrad [PL/GB]; GB (UsOnly)
GAIT, Michael, J. [GB/GB]; GB (UsOnly)
Inventors:
MISIURA, Konrad; GB
GAIT, Michael, J.; GB
Agent:
GAUNT, Robert, John; Stevens, Hewlett & Perkins 5 Quality Court Chancery Lane London WC2A 1HZ, GB
PENNANT, Pyers; Stevens, Hewlett & Perkins 1 Serjeants' Inn Fleet Street London EC4Y 1LL, GB
Priority Data:
9009980.503.05.1990GB
Title (EN) PHOSPHORAMIDITE DERIVATIVES, THEIR PREPARATION AND THE USE THEREOF IN THE INCORPORATION OF REPORTER GROUPS ON SYNTHETIC OLIGONUCLEOTIDES
(FR) DERIVES DE PHOSPHORAMIDITE, LEUR PREPARATION ET UTILISATION DANS L'INCORPORATION DE GROUPES REPORTEURS SUR DES OLIGONUCLEOTIDES SYNTHETIQUES
Abstract:
(EN) Phosphoramidite derivatives of formulae (V) or (XII), wherein X is a reporter group and Y and Z are protecting groups. The reporter group X may comprise any hapten or other detectable moiety and examples include boitin, dinitrophenyl, dansyl and fluoresceinyl. There may be a linker arm, of variable length, between the reporter group and the rest of the molecule. Examples of the protecting groups, Y and Z, include 4,4'-dimethoxytrityl, trifluoroacetyl and fluorenylmethoxycarbonyl (Fmoc). The phosphoramidite derivatives are useful in the single or multiple labelling of synthetic oligonucleotides. Processes for the preparation of these phosphoramidite derivatives are also disclosed.
(FR) Des dérivés de phosphoramidite ayant les formules (V) ou (XII), où X représente un groupe reporteur et Y et Z représentent des groupes protecteurs. Le groupe reporteur X peut comprendre n'importe quel haptène ou fraction détectable par exemple la biotine, le dinitrophényle, le dansyle et le fluorescéinyle. Il peut y avoir une branche de liaison, de longueur variable entre le groupe reporteur et le reste de la molécule. Des exemples des groupes protecteurs Y et Z comprennent le 4, 4'-diméthoxytrityle, le trifluoroacétyle et le fluorénylméthoxycarbonyle (Fmoc). Les dérivés de phosphoramidite sont utiles au marquage simple ou multiple d'oligonucléotides synthétiques. Des procédés de préparation de ces dérivés de phosphoramidite sont aussi décrits.
Designated States: CA, JP, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0527184US5567811US5864032JPH05507074CA2081320DE000069111807