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1. (WO1991009310) FREE FATTY ACID DETERMINATION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/009310 International Application No.: PCT/US1990/007202
Publication Date: 27.06.1991 International Filing Date: 07.12.1990
IPC:
C12N 15/62 (2006.01) ,G01N 33/92 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
62
DNA sequences coding for fusion proteins
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
92
involving lipids, e.g. cholesterol
Applicants:
LIDAK PHARMACEUTICALS [US/US]; 11077 No. Torrey Pines Rd. La Jolla, CA 92037, US
Inventors:
KLEINFELD, Alan, M.; US
Agent:
HUBBARD, Grant, L. ; Nelson, Hubbard & Roediger 2623 North Seventh Street Phoenix, AZ 85006, US
Priority Data:
449,97212.12.1989US
Title (EN) FREE FATTY ACID DETERMINATION
(FR) DETERMINATION D'ACIDE GRAS LIBRE
Abstract:
(EN) A method for determining free fatty acids comprising the steps of mixing a solution suspected of containing free fatty acid with a reagent comprising a fluorescently modified fatty acid binding protein, detecting a change in fluorescence of the fluorescently modified fatty acid binding protein, and relating said change in fluorescence to the amount of free fatty acid in the solution is disclosed.
(FR) Procédé de détermination d'acide gras libre comprenant les étapes consistant à mélanger une solution dont on soupçonne qu'elle contient un acide gras libre, avec un réactif comprenant une protéine de liaison d'acide gras modifiée par fluorescence, à détecter un changement de fluorescence de la protéine de liaison d'acide gras modifiée par fluorescence et à corréler ledit changement de fluorescence avec la quantité d'acide gras libre se trouvant dans la solution.
Designated States: AU, JP
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0457901AU1991071629