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1. WO1991009137 - RECOMBINANT THYROTROPIN RECEPTOR

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CLAIHS

What is claimed is:

1. Recombinant, enzymatically active thyrotropin receptor.

2. A DNA sequence comprising the DNA sequence of Figure 1.

3. A amino acid sequence comprising the nucleotide sequence of Figure 1.

4. A plasmid comprising the DNA sequence of claim 2.

5. A host cell transformed by the plasmid of claim 4.

6. A method of producing thyrotropin receptor, comprising culturing the transformed cell of claim 5, under conditions allowing expression of thyrotropin receptor, and recovering said thyrotropin receptor.

7. An antibody against the recombinant thyrotropin receptor of claim 1.

8. The antibody of claim 7, wherein said antibody is selected from the group consisting of a monoclonal antibody, polyclonal antibody, an anti- idiotypic antibody, or an anti anti-idiotypic antibody.

9. Recombinant enzymatically active thyrotropin receptor wherein a 50 amino acid region comprising

* residues 317-366 has been deleted.

* 10. An antibody against the recombinant 5 thyrotropin receptor of claim 9.

11. The antibody of claim 10, wherein, said antibody is selected from a group consisting of a monoclonal antibody, polyclonal antibody, an anti- idiotypic antibody, or an anti anti-idiotypic

10 antibody.

12. A recombinant thyrotropin receptor peptide comprised of the eight amino acid region comprising residues 38-45 of the thyrotropin receptor of claim 1.

13. An antibody against the recombinant 15 thyrotropin receptor peptide of claim 12.

14. The antibody of claim 13 wherein, said antibody is selected from a group consisting of a monoclonal antibody, polyclonal antibody, an anti- idiotypic antibody, or an anti anti-idiotypic 0 antibody.

15. Recombinant thyrotropin receptor wherein an eight amino acid region comprising residues 38-45 have

, been deleted or replaced.

* 16. A recombinant thyrotropin receptor peptide 5 comprised of the mid-region domain C comprising amino acid residues 171-260 of the thyrotropin receptor of claim 1.

17. An antibody against the recombinant thyrotropin receptor peptide of claim 16.

18. The antibody of claim 17, wherein said antibody is selected from a group consisting of a monoclonal antibody, polyclonal antibody, an antiidiotypic antibody, or an anti anti-idiotypic antibody.

19. Recombinant thyrotropin receptor wherein the mid-region domain C comprising amino acid residues 171-260 has been deleted or replaced.

20. A method of detecting thyrotropin receptor in a sample comprising contacting said sample with the antibody of any of claims 7 or 8, wherein said antibody is detectably labeled, so as to form a complex between said thyrotropin receptor in said sample and said detectably labeled antibody, and detecting the complexed or uncomplexed detectably labeled antibody.

21. A kit for the detection of thyrotropin receptor in a sample, comprising container means comprising one or more containers, wherein one of said containers comprises detectably labeled antibody against thyrotropin receptor.

22. A method of detecting an antibody to thyrotropin receptor in a sample comprising contacting said sample with the thyrotropin receptor of claim l, wherein said thyrotropin receptor is detectably labeled, so as to form a complex between the antibody to said thyrotropin receptor in said sample and said detectably labeled thyrotropin receptor, and detecting the complexed or uncomplexed detectably labeled 4 thyrotropin receptor.

s, 23. A method for detecting an antibody to

5 thyrotropin in a sample, comprising contacting said sample with Chinese Hamster ovary (CHO) cells stably transfected with human thyrotropin receptor cDNA wherein said CHO cells are expressing the functional thyrotropin receptor of claim 1, wherein said 0 thyrotropin receptor is detectably labeled so as to form a complex between the antibody to said thyrotropin receptor in said sample and said detectably labeled thyrotropin receptor, and detecting the complexed or uncomplexed detectably labeled 5 thyrotropin receptor.

24. A method of differentiating between autoimmune and non-autoimmune forms of thyrotoxicosis comprising contacting a sample with the antibody of any of claims 7 or 8.

0 25. The method of claim 24, wherein said antibody is detectably labeled, so as to form a complex between said thyrotropin receptor in said sample and said detectably labeled antibody and detecting the complexed or uncomplexed detectably 5 labeled antibody.
*
26. A kit for the detection of an antibody to

* thyrotropin receptor in a sample, comprising container means comprising one or more containers, wherein one of said containers comprises detectably labeled 0 thyrotropin receptor.

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27. A pharmaceutical preparation comprising the recombinant thyrotropin receptor of claim 1.

28. A method of treating thyrotoxicosis with the pharmaceutical preparation of claim 13.

5 29. A T cell which is specific for the autoimmune T-cell receptor for thyrotropin receptor.

30. A peptide which is specific for the autoimmune T-cell receptor for thyrotropin receptor.

31. A suppressor T cell specific for anti-10 thyrotropin receptor auto-antibody.

32. A pharmaceutical preparation comprising the
T cell, peptide, or suppressor T cell of claims 29-31.

33. A method of treating thyrotoxicosis with the pharmaceutical preparation of claim 32.

15 34. A pharmaceutical preparation comprising the recombinant thyrotropin receptor peptide of claim 12.

35. A method of treating thyrotoxicosis with the pharmaceutical preparation of claim 34.

36. A pharmaceutical preparation comprising the 20 recombinant thyrotropin receptor peptide of claim 16.

37. A method of treating thryotoxicisis with the pharmaceutical preparation of claim 36.