Search International and National Patent Collections
Some content of this application is unavailable at the moment.
If this situation persists, please contact us atFeedback&Contact
1. (WO1991009137) RECOMBINANT THYROTROPIN RECEPTOR
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/009137 International Application No.: PCT/US1990/007387
Publication Date: 27.06.1991 International Filing Date: 19.12.1990
Chapter 2 Demand Filed: 18.07.1991
IPC:
A61K 38/00 (2006.01) ,C07K 14/72 (2006.01) ,C07K 16/28 (2006.01) ,G01N 33/564 (2006.01) ,G01N 33/76 (2006.01)
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38
Medicinal preparations containing peptides
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
705
Receptors; Cell surface antigens; Cell surface determinants
72
for hormones
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18
against material from animals or humans
28
against receptors, cell surface antigens or cell surface determinants
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
564
for pre-existing immune complex or autoimmune disease
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
74
involving hormones
76
Human chorionic gonadotropin
Applicants:
RAPOPORT, Basil [US/US]; US
Inventors:
RAPOPORT, Basil; US
Agent:
GOLDSTEIN, Jorge, A. ; Sterne, Kessler, Goldstein & Fox 1225 Connecticut Avenue, N.W. Suite 300 Washington, DC 20036, US
Priority Data:
451,97320.12.1989US
575,01830.08.1990US
Title (EN) RECOMBINANT THYROTROPIN RECEPTOR
(FR) RECEPTEUR DE THYROTROPINE RECOMBINANT
Abstract:
(EN) Functionally-active, human thyrotropin receptor has been expressed in non-thyroidal eukaryotic cells. A human thyroid cDNA library was screened with two synthetic oligonucleotides based on the reported amino acid sequence of the 3rd and 4th transmembrane domains of a putative human thyrotropin receptor and related receptors. The nucleotide sequence of a 4 kb clone revealed an open reading frame of 764 amino acids (86,816 Daltons) with a putative signal peptide, seven transmembrane domains, five potential glycosylation sites, and a very short intracytoplasmic region. Homology with the extracellular domain of the pig LH/CG receptor was only 33 %. Chinese hamster ovary cells stably transfected with this cDNA in an expression vector generated a functional receptor, able to activate adenylate cyclase, specifically in response to thyrotropin stimulation.
(FR) L'invention se rapporte à un récepteur de thyrotropine humaine fonctionnellement actif qu'on a produit par expression à l'intérieur de cellules eukaryotiques non thyroïdiennes. On a soumis une bibliothèque d'ADNc de thyroïde humaine à une opération de triage au moyeu de deux oligonucléotides synthétiques sur la base de la séquence d'acides aminés rapportée des troisième et quatrième domaines transmembranes et d'un récepteur de thyrotropine humaine putatif et de récepteurs associés. La séquence de nucléotides d'un clone de 4 kb a révélé une structure de lecture ouverte de 764 acides aminés (86.816 daltons) avec un peptide de signal putatif, sept domaines transmembrane, cinq sites de glycosylation potentiels et une région intracytoplasmique trés courte. La part d'homologie avec le domaine extracellulaire du récepteur LH/CG du cochon s'est située à 33 % seulement. Des cellules d'ovaire d'hamster chinois, transfectées de façon stable avec cet ADNc dans un vecteur d'expression, on généré un récepteur fonctionnel, capable d'activer l'adénylate cyclase, particulièrement en réponse à une stimulation de thyrotropine.
Designated States: AU, BB, BG, BR, CA, FI, HU, JP, KP, KR, LK, MC, MG, MW, NO, RO, SD, SU, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CM, GA, ML, MR, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0506890JPH05504683AU1991072573