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1. (WO1991009132) CLONING VECTOR FOR USE IN LACTIC ACID BACTERIA AND A METHOD FOR CONSTRUCTING THE SAME
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/009132 International Application No.: PCT/DK1990/000337
Publication Date: 27.06.1991 International Filing Date: 20.12.1990
Chapter 2 Demand Filed: 26.06.1991
IPC:
C07K 14/335 (2006.01) ,C12N 15/74 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
335
from Lactobacillus (G)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
Applicants:
VALIO FINNISH COOPERATIVE DAIRIES ASSOCIATION [FI/FI]; Kalevankatu 56 SF-00181 Helsinki, FI (AllExceptUS)
WESSELS, Stephen [DK/DK]; DK
JOSEPHSEN, Jytte [DK/DK]; DK
VOGENSEN, Finn [DK/DK]; DK
NIELSEN, Egil, Waagner [DK/DK]; DK
VON WRIGHT, Atte [FI/FI]; FI (UsOnly)
TYNKKYNEN, Soile [FI/FI]; FI (UsOnly)
Inventors:
WESSELS, Stephen; DK
JOSEPHSEN, Jytte; DK
VOGENSEN, Finn; DK
NIELSEN, Egil, Waagner; DK
VON WRIGHT, Atte; FI
TYNKKYNEN, Soile; FI
Agent:
PLOUGMANN & VINGTOFT; Sankt Annæ Plads 11 P.O. Box 3007 DK-1021 Copenhagen K, DK
Priority Data:
PCT/DK89/0029820.12.1989AT
Title (EN) CLONING VECTOR FOR USE IN LACTIC ACID BACTERIA AND A METHOD FOR CONSTRUCTING THE SAME
(FR) VECTEUR DE CLONAGE UTILISE DANS DES BACTERIES D'ACIDE LACTIQUE ET SON PROCEDE D'ELABORATION
Abstract:
(EN) A recombinant plasmid useful as a cloning vector in lactic acid bacteria and only containing DNA of lactic acid bacterial origin, the plasmid comprising (a) a DNA fragment comprising a replication region functional in lactic acid bacteria and (b) a DNA fragment comprising a marker gene selectable in lactic bacteria, the expression of which allows one-step primary selection in lactic acid bacterial cells transformed with the recombinant plasmid; a method for constructing the cloning vector plasmid; the recombinant plasmid with an inserted gene coding for a desired gene product; a method of preparing an improved food starter culture by introducing the recombinant plasmid with an inserted gene coding for a desired gene product and food starter cultures prepared by the method are provided.
(FR) L'invention concerne un plasmide de recombinaison utile en tant que vecteur de clonage dans des bactéries d'acide lactique et ne contenant que de l'ADN d'origine bactérienne d'acide lactique, le plasmide comprenant (a) un fragment d'ADN comportant une région de réplication fonctionnelle dans des bactéries d'acide lactique, et (b) un fragment d'ADN comprenant un gène marqueur pouvant être sélectionné parmi des bactéries lactiques, dont l'expression permet la sélection primaire en une étape dans des cellules bactériennes d'acide lactique transformées à l'aide du plasmide de recombinaison; un procédé d'élaboration du plasmide du vecteur de clonage; le plasmide de recombinaison comportant un gène inséré codant pour un produit de gène voulu; un procédé de préparation d'un bouillon de culture d'amorce alimentaire amélioré, par introduction du plasmide de recombinaison à l'aide d'un gène inséré codant pour un produit de gène voulu, ainsi que des bouillons de culture d'amorces alimentaires préparés selon le procédé.
Designated States: AU, BR, CA, JP, NO, SU, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0506789US5580787CA2072007AU1991070459