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1. (WO1991009050) NOVEL ORGAN-SPECIFIC PLANT PROMOTER SEQUENCES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/009050 International Application No.: PCT/US1990/007231
Publication Date: 27.06.1991 International Filing Date: 07.12.1990
Chapter 2 Demand Filed: 08.07.1991
IPC:
C12N 9/00 (2006.01) ,C12N 15/82 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
Applicants:
THE TRUSTEES OF ROCKEFELLER UNIVERSITY [US/US]; 1230 York Avenue New York, NY 10021, US
Inventors:
EDWARDS, Janice, W.; US
WALKER, Elsbeth, L.; US
CORUZZI, Gloria; US
Agent:
MISROCK, S., Leslie; Pennie & Edmonds 1155 Avenue of the Americas New York, NY 10036, US
Priority Data:
448,03608.12.1989US
Title (EN) NOVEL ORGAN-SPECIFIC PLANT PROMOTER SEQUENCES
(FR) SEQUENCES PROMOTRICES DE PLANTES A TRANSCRIPTION SPECIFIQUE D'ORGANES
Abstract:
(EN) The present invention relates to novel plant organ-specific transcriptional promoter nucleic acid sequences, which regulate the expression of glutamine synthetase isoenzymes. Specifically, promoter sequences were isolated from the nuclear gene for chloroplast GS2 glutamine synthetase and from two nuclear genes for cytosolic GS3 glutamine synthetase in the pea plant, $i(Pisum sativum). Accordingly, the present invention provides for the nucleic acid sequences of the GS2, GS3A and GS3B promoter sequences as well as functional portions thereof. The invention further provides for promoters homologous to GS2, GS3A and GS3B, gene fusions comprising the novel glutamine synthetase promoters, and transgenic plants which comprise the promoters of the invention.
(FR) La présente invention se rapporte à des séquences promotrices d'acide nucléique de plantes à transcription spécifique d'organes, qui régulent l'expression des isoenzymes de synthèse de glutamine. Plus particulièrement, on a isolé les séquences promotrices à partir du gène nucléaire pour la synthèse de glutamine GS2 et à partir de deux gènes nucléaires pour la synthèse de glutamine GS3 cytosolique dans le pois $i(Pisum sativum). Par conséquence, la présente invention permet l'obtention des séquences d'acide nucléique des séquences promotrices GS2, GS3A et GS3B aussi bien que des portions fonctionnelles de celles-ci. De plus la présente invention permet l'obtention de promoteurs homologues aux GS2, GS3A et GS3B, des fusions de gènes comprenant les promoteurs de synthèse de glutamine nouveaux et de plantes transgéniques comprenant les promoteurs de l'invention.
Designated States: AU, BB, BG, BR, CA, FI, HU, JP, KR, LK, MC, MG, MW, NO, RO, SD, SU
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CM, GA, ML, MR, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AU1991071654