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1. (WO1991008308) A NEW METHOD FOR DETECTING A SPECIFIC NUCLEIC ACID SEQUENCE IN A SAMPLE OF CELLS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/008308 International Application No.: PCT/US1990/006953
Publication Date: 13.06.1991 International Filing Date: 29.11.1990
Chapter 2 Demand Filed: 28.06.1991
IPC:
C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01) ,C12Q 1/70 (2006.01) ,G01N 33/569 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
70
involving virus or bacteriophage
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
569
for micro-organisms, e.g. protozoa, bacteria, viruses
Applicants:
PHARMACIA GENETIC ENGINEERING, INC. [US/US]; 1028 North Torrey Pines Road La Jolla, CA 92037, US
Inventors:
FROSTELL, Asa; SE
NUNN, Michael, F.; US
Agent:
FITTING, Thomas ; Dressler, Goldsmith, Shore, Sutker & Milnamow, Ltd. 11300 Sorrento Valley Road, Suite 200 San Diego, CA 92121, US
Priority Data:
443,91030.11.1989US
505,83306.04.1990US
548,02705.07.1990US
Title (EN) A NEW METHOD FOR DETECTING A SPECIFIC NUCLEIC ACID SEQUENCE IN A SAMPLE OF CELLS
(FR) NOUVELLE METHODE PERMETTANT DE DETECTER UNE SEQUENCE D'ACIDE NUCLEIQUE SPECIFIQUE A PARTIR D'UN PRELEVEMENT DE CELLULES
Abstract:
(EN) The present invention provides a method for releasing genomic DNA from cells. The method can be combined with a method for performing a polymerase chain reaction to provide a simple, rapid procedure for assaying for the presence of a specific nucleic acid sequence in a sample of genomic DNA-containing cells.
(FR) Le présente invention fait appel à une méthode permettant de libérer de l'ADN génomique à partir des cellules. On peut combiner cette méthode à une méthode permettant de réaliser une réaction en chaîne de polymérase de façon à établir une procédure simple et rapide permettant de détecter la présence d'une séquence d'acide nucleïque spécifique à partir d'un prelèvement de cellules contenant de l'ADN génomique.
Designated States: JP
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0504278JPH05504475