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1. (WO1991006678) DNA SEQUENCING
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/006678 International Application No.: PCT/US1990/006178
Publication Date: 16.05.1991 International Filing Date: 26.10.1990
IPC:
C07H 21/00 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 35/00 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35
Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/-G01N33/148; Handling materials therefor
Applicants:
SRI INTERNATIONAL [US/US]; 333 Ravenswood Avenue Menlo Park, CA 94025-3493, US
TSIEN, Roger, Y. [US/US]; US
Inventors:
TSIEN, Roger, Y.; US
ROSS, Pepi; US
FAHNESTOCK, Margaret; US
JOHNSTON, Allan, J.; US
Agent:
CHEN, John, Y.; SRI International 333 Ravenswood Avenue Menlo Park, CA 94025-3493 , US
Priority Data:
427,32126.10.1989US
Title (EN) DNA SEQUENCING
(FR) SEQUENÇAGE D'ADN
Abstract:
(EN) The present invention relates to an instrument and a method to determine the nucleotide sequence in a DNA molecule without the use of a gel electrophoresis step. The method employs an unknown primed single stranded DNA sequence which is immobilized or entrapped within a chamber with a polymerase so that the sequentially formed cDNA can be monitored at each addition of a blocked nucleotide by measurement of the presence of an innocuous marker on specified deoxyribonucleotides. The invention also relates to a method of determining the unknown DNA nucleotide sequence using blocked deoxynucleotides. The blocked dNTP has an innocuous marker so that its identity can be easily determined. The present instrument and method provide a rapid accurate determination of a DNA nucleotide sequence without the use of gel electrophoresis.
(FR) Instrument et procédé permettant de déterminer la séquence de nucléotides se trouvant dans une molécule d'ADN, sans l'emploi d'une étape d'électrophorèse sur gel. Le procédé emploie une séquence d'ADN monocaténaire amorçée inconnue, laquelle est immobilisée ou piégée à l'intérieur d'une chambre à l'aide d'une polymérase, de sorte que l'ADN complémentaire formé de manière séquentielle peut être contrôlé à chaque addition d'un nucléotide bloqué, par mesure de la présence d'un marqueur inoffensif sur des désoxyribonucléotides spécifiés. L'invention concerne également un procédé de détermination de la séquence de nucléotides d'ADN inconnue à l'aide de désoxynucléotides bloqués. Le dNTP (désoxyribonucléotide-triphosphate) bloqué comporte un marqueur inoffensif, de sorte que son identité peut être facilement déterminée. L'instrument et les procédés de l'invention permettent d'obtenir une détermination précise et rapide d'une séquence de nucléotides d'ADN sans électrophorèse sur gel.
Designated States: CA, JP
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0450060CA2044616