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1. (WO1991006677) RECEPTOR INFECTION ASSAY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/006677 International Application No.: PCT/US1990/006129
Publication Date: 16.05.1991 International Filing Date: 23.10.1990
Chapter 2 Demand Filed: 22.05.1991
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
THE SALK INSTITUTE FOR BIOLOGICAL STUDIES [US/US]; 10010 North Torrey Pines Road La Jolla, CA 92037, US
Inventors:
WEINBERGER, Carry, Alan; US
Agent:
CAMPBELL, Cathryn ; Pretty, Schroeder, Brueggemann & Clark 444 South Flower Street Suite 2000 Los Angeles, CA 90071, US
Priority Data:
426,89425.10.1989US
Title (EN) RECEPTOR INFECTION ASSAY
(FR) ANALYSE DE RECEPTEUR PAR INFECTION
Abstract:
(EN) The presente invention relates to improvements in a bioassay useful for identifying materials that function as ligands for a hormone or hormone-like receptor protein measured by means of induction of reporter DNA driven by a promoter of a hormone response element. The improvement comprises producing a cell operatively harboring DNA encoding reporter gene and receptor gene by coinfection of a mammalian cell with a viral expression vector. The expedient of this invention permits commercial realization and exploitation of the bioassay system.
(FR) L'invention concerne des améliorations à une bioanalyse utile pour identifier des matériaux qui fonctionnent comme ligands pour une hormone ou une protéine de récepteur semblable à une hormone mesurée par induction d'ADN rapporteur entraîné par un promoteur d'un élément de réponse hormonale. L'amélioration consiste à produire un gène rapporteur de codage d'ADN dans la cellule et un gène récepteur par co-infection d'une cellule mammifère avec un vecteur d'expression virale. Cette invention permet la réalisation et l'exploitation commerciale du système de bioanalyse.
Designated States: AU, CA, JP
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0497894JPH05504254CA2067216AU1990067397