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1. (WO1991006667) PRODUCTION OF PROTEINS USING HOMOLOGOUS RECOMBINATION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/006667 International Application No.: PCT/US1990/006436
Publication Date: 16.05.1991 International Filing Date: 06.11.1990
IPC:
C07K 14/505 (2006.01) ,C12N 15/85 (2006.01) ,C12N 15/86 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
475
Growth factors; Growth regulators
505
Erythropoietin (EPO)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
86
Viral vectors
Applicants:
CELL GENESYS, INC. [US/US]; 344 Lakeside Drive Foster City, CA 94404, US
Inventors:
SKOULTCHI, Arthur, I.; US
Agent:
ROWLAND, Bertram, I. ; Cooley, Godward, Castro, Huddleson & Tatum Five Palo Alto Square, 4th Floor Palo Alto, CA 94306, US
Priority Data:
432,06906.11.1989US
Title (EN) PRODUCTION OF PROTEINS USING HOMOLOGOUS RECOMBINATION
(FR) PRODUCTION DE PROTEINES PAR RECOMBINAISON HOMOLOGUE
Abstract:
(EN) Methods and compositions are provided for expression of mammalian genes in culture. An amplifiable gene is introduced by homologous recombination in juxtaposition to a target gene, the resulting combination of amplifiable gene and target gene transferred to a convenient host and the target gene amplified by means of the amplifiable gene. The resulting expression host may then be grown in culture with enhanced expression of the target gene.
(FR) Des procédés et des compositions sont utiles pour exprimer des gènes de mammifères en culture. On introduit un gène amplifiable par recombinaison homologue, juxtaposé à un gène cible, on transfère la combinaison ainsi obtenue de gène amplifiable et de gène cible à un hôte approprié et on amplifie le gène cible au moyen du gène amplifiable. On peut ensuite cultiver l'hôte d'expression ainsi obtenu, de manière à accroître l'expression du gène cible.
Designated States: AU, CA, JP, NO
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
NO19912642SG122763SG88714ES2090297EP0452484EP0747485
EP0945515US20040265860JP2001186897 CA2045175DE000069027526DE000069032809
KR1002337810000*AU1991077407DK0747485DK0452484