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1. (WO1991006655) E. COLI SECRETORY STRAINS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/006655 International Application No.: PCT/US1989/004788
Publication Date: 16.05.1991 International Filing Date: 31.10.1989
Chapter 2 Demand Filed: 06.05.1991
IPC:
C07K 14/245 (2006.01) ,C07K 14/54 (2006.01) ,C07K 16/24 (2006.01) ,C12N 15/70 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
24
from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
245
Escherichia (G)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
52
Cytokines; Lymphokines; Interferons
54
Interleukins (IL)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18
against material from animals or humans
24
against cytokines, lymphokines or interferons
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70
Vectors or expression systems specially adapted for E. coli
Applicants:
SCHERING CORPORATION [US/US]; 2000 Galloping Hill Road Kenilworth, NJ 07033, US
Inventors:
LUNN, Charles, A.; US
NARULA, Satwant, K.; US
REIM, Richard, L.; US
Agent:
DULAK, Norman, C. ; Schering-Plough Corporation One Giralda Farms Madison, NJ 07940-1000, US
Priority Data:
Title (EN) E. COLI SECRETORY STRAINS
(FR) SOUCHES D'E. COLI SECRETANT DES PROTEINES
Abstract:
(EN) Strains of $i(E. coli) are provided which are capable of secreting heterologous recombinant proteins through the outer cell wall and directly into the culture medium. Methods are also provided for making and identifying such strains, and for using them to make heterologous proteins that have high biological activity, without the need for cell disruption.
(FR) Souches de bactérie $i(Escherichia coli) capables de secréter des protéines recombinantes hétérologues au travers de la paroi cellulaire externe et directement dans le milieu de culture. La présente invention décrit aussi des méthodes de production et d'identification de ces souches, ainsi que des méthodes d'utilisation desdites souches en vue de la fabrication de protéines hétérologues à haute activité biologique, rendant inutile la disruption cellulaire.
Designated States: AU, BB, BG, BR, DK, FI, HU, JP, KP, KR, LK, MC, MG, MW, NO, RO, SD, SU
European Patent Office (AT, BE, CH, DE, FR, GB, IT, LU, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CM, GA, ML, MR, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
FI921874NO19921674EP0497757DE000068916444KR10199600131343AU1989046447