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1. (WO1991006634) IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/006634 International Application No.: PCT/US1990/006449
Publication Date: 16.05.1991 International Filing Date: 07.11.1990
Chapter 2 Demand Filed: 07.06.1991
IPC:
C12N 9/02 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
Applicants:
BIO-TECHNOLOGY GENERAL CORP. [US/US]; 1250 Broadway 20th Floor New York, NY 10001, US
Inventors:
BARTFELD, Daniel; CA
LIFSHITZ, Ruth; IL
HADARY, Dany; CA
Agent:
WHITE, John, P.; Cooper & Dunham 30 Rockefeller Plaza New York, NY 10112, US
Priority Data:
432,87107.11.1989US
Title (EN) IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS
(FR) PROCEDE AMELIORE DE PURIFICATION DE DISMUTASE RECOMBINANTE DE SUPEROXYDE DE CUIVRE/ZINC (CU-ZN) A PARTIR DE BACTERIES OU DE CELLULES EUCARYOTES
Abstract:
(EN) The subject invention provides a method for recovering a solution containing purified, enzymatically active Cu-Zn superoxide dismutase or a polypeptide analog thereof having substantially the same amino acid sequence as, and the biological activity of, naturally-occurring Cu-Zn superoxide dismutase from a composition which comprises cells containing Cu-Zn superoxide dismutase or a polypeptide analog thereof. The invention also provides a method of increasing the yield of recovered solutions having an increased concentration of b isoform of an enzymatically-active polypeptide analog of Cu-Zn superoxide dismutase from a composition which comprises cells containing a, b and c isoforms of the polypeptide analog.
(FR) Un procédé permet de récupérer une solution contenant de la dismutase purifiée enzymatiquement active de superoxyde de Cu-Zn ou un analogue polypeptidique de celle-ci ayant essentiellement la même séquence d'acides aminés et la même activité biologique de la dismutase de superoxyde de Cu-Zn naturelle, à partir d'une composition qui comprend des cellules contenant de la dismutase de superoxyde de Cu-Zn ou un analogue polypeptidique de celle-ci. Ce procédé permet en outre d'augmenter le rendement d'extraction des solutions ayant une concentration accrue de l'isoforme b d'un analogue polypeptidique enzymatiquement actif de la dismutase de superoxyde de Cu-Zn à partir d'une composition qui comprend des cellules contenant des isoformes a, b et c de l'analogue polypeptidique.
Designated States: AU, BR, CA, FI, HU, JP, KR, NO, SU
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
IL96136EP0499621CA2073043AU1991068718