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1. (WO1991006626) TRIPLE HELIX FORMATION IN OLIGONUCLEOTIDE THERAPY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/006626 International Application No.: PCT/US1990/006128
Publication Date: 16.05.1991 International Filing Date: 23.10.1990
Chapter 2 Demand Filed: 07.05.1991
IPC:
C12N 15/11 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
GILEAD SCIENCES, INC. [US/US]; 344 Lakeside Drive Foster City, CA 94404, US (AllExceptUS)
FROEHLER, Brian [US/US]; US (UsOnly)
TOOLE, John, J. [US/US]; US (UsOnly)
Inventors:
FROEHLER, Brian; US
TOOLE, John, J.; US
Agent:
MURASHIGE, Kate, H. ; Irell & Manella 545 Middlefield Road Suite 200 Menlo Park, CA 94025, US
Priority Data:
425,80323.10.1989US
502,27229.03.1990US
559,95830.07.1990US
Title (EN) TRIPLE HELIX FORMATION IN OLIGONUCLEOTIDE THERAPY
(FR) FORMATION D'UNE TRIPLE CHAINE HELICOIDALE EN THERAPIE OLIGONUCLEOTIDIQUE
Abstract:
(EN) Oligonucleotides having tandem sequences of inverted polarity, i.e., oligonucleotides comprising regions of the formula: 3'-----5'--C--5'-----3' or 5'-----3'--C--3'-----5', wherein -C- symbolizes any method of coupling the nucleotide sequence of opposite polarity, are useful for forming an extended triple helix with a double-helical nucleotide duplex. Single or mixed motif oligomers may be used. The inverted polarity also stabilizes the single-strand oligonucleotides to exonuclease degradation.
(FR) Des oligonucléotides possédant des séquences en tandem à polarité inversée, c'est-à-dire des oligonucléotides comprenant des régions dont la formule est: 3'-----5'--C--5'-----3' ou 5'-----3'--C--3'-----5', où -C- symbolise tout procédé de couplage de la séquence nucléotidique à polarité opposée, sont utiles à la formation d'une triple chaîne hélicoïdale possédant un duplex nucléotidique à double hélice. On peut utiliser des oligomères à motifs simples ou mélangés. La polarité inversée sert également à rendre les oligonucléotides monocaténaires résistants à la dégradation par exonucléase.
Designated States: AU, CA, JP, KR, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0539371CA2071536AU1991071481