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1. (WO1991005861) NON-SPECIFIC DNA AMPLIFICATION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/005861 International Application No.: PCT/US1990/005938
Publication Date: 02.05.1991 International Filing Date: 16.10.1990
Chapter 2 Demand Filed: 15.05.1991
IPC:
C07K 14/08 (2006.01) ,C07K 14/535 (2006.01) ,C07K 14/54 (2006.01) ,C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005
from viruses
08
RNA viruses
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
52
Cytokines; Lymphokines; Interferons
53
Colony-stimulating factor (CSF)
535
Granulocyte CSF; Granulocyte-macrophage CSF
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
52
Cytokines; Lymphokines; Interferons
54
Interleukins (IL)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
GENELABS INCORPORATED [US/US]; 505 Penobscot Drive Redwood City, CA 94063, US
Inventors:
LOVETT, Michael; US
REYES, Gregory, R.; US
WEISSMAN, Sherman, M.; US
JORGENSON, Linda, M.; US
Agent:
DEHLINGER, Peter, J.; Dehlinger & Swiss 350 Cambridge Avenue Suite 100 Palo Alto, CA 94306, US
GOLDIN, Douglas, Michael; J.A. Kemp & Co. 14 South Square Gray's Inn London WC1R 5EU, GB
Priority Data:
422,39216.10.1989US
Title (EN) NON-SPECIFIC DNA AMPLIFICATION
(FR) AMPLIFICATION NON SPECIFIQUE D'ADN
Abstract:
(EN) A method of non-specifically amplifying different-sequence fragments in a mixture of duplex DNA fragments is disclosed. The fragments are provided with end linkers, and the mixture is amplified by successive primer-initiated replication. Also disclosed is a method of cloning cDNA species which are homologous to a region of contiguous genomic DNA and are selected from a mixture of cDNA species.
(FR) On a mis au point un procédé d'amplification non spécifique de fragments de séquences différentes dans un mélange de fragments d'ADN duplex. Les fragments sont dotés de liaisons terminales, et onamplifie le mélange par réplications successives déclenchées par une amorce. On a également mis au point un procédé de clonage d'espèces d'ADN complémentaire, lesquelles sont homologues à une région d'ADN génomique contiguë, et sont sélectionnées dans un mélange d'espèces d'ADN complémentaire.
Designated States: AU, CA, JP, KR
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0495911JPH05508305CA2067363AU1990066461