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1. (WO1991002814) NUCLEIC ACID AMPLIFICATION PROCESS

Pub. No.:    WO/1991/002814    International Application No.:    PCT/EP1989/000981
Publication Date: Mar 7, 1991 International Filing Date: Aug 19, 1989
IPC: C12Q 1/68
C12Q 1/70
Applicants: AKZO N.V.
Inventors: MALEK, Lawrence, T.
DAVEY, Cheryl
Title: NUCLEIC ACID AMPLIFICATION PROCESS
Abstract:
This invention relates to a process for amplifying a specific nucleic acid sequence. The process involves synthesizing single-stranded RNA, single-stranded DNA and double-stranded DNA. The single-stranded RNA is a first template for a first primer, the single-stranded DNA is a second template for a second primer, and the double-stranded DNA is a third template for synthesis of a plurality of copies of the first template. A sequence of the first primer or the second primer is complementary to a sequence of the specific nucleic acid and a sequence of the first primer or the second primer is homologous to a sequence of the specific nucleic acid. The amplification process may be used to increase the quantity of the specific nucleic acid sequence to allow detection, or to increase the purity of the specific nucleic acid sequence as a substitute for conventional cloning methodology.