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1. (WO1991002049) AN INTEGRATED CELL CULTURE-PROTEIN PURIFICATION SYSTEM FOR THE AUTOMATED PRODUCTION AND PURIFICATION OF CELL CULTURE PRODUCTS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1991/002049 International Application No.: PCT/US1990/004361
Publication Date: 21.02.1991 International Filing Date: 03.08.1990
IPC:
C12M 1/00 (2006.01) ,C12M 1/12 (2006.01) ,C12M 1/36 (2006.01) ,C12M 3/06 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
12
with sterilisation, filtration, or dialysis means
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
36
including condition or time responsive control, e.g. automatically controlled fermentors
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3
Tissue, human, animal or plant cell, or virus culture apparatus
06
with filtration, ultrafiltration, inverse osmosis or dialysis means
Applicants:
GRANDICS, Peter [HU/US]; US
SZATHMARY, Susan [US/US]; US
Inventors:
GRANDICS, Peter; US
SZATHMARY, Susan; US
Priority Data:
389,60604.08.1989US
Title (EN) AN INTEGRATED CELL CULTURE-PROTEIN PURIFICATION SYSTEM FOR THE AUTOMATED PRODUCTION AND PURIFICATION OF CELL CULTURE PRODUCTS
(FR) SYSTEME INTEGRE DE CULTURE DE CELLULES-PURIFICATION DE PROTEINES POUR LA PRODUCTION ET LA PURIFICATION AUTOMATISEES DE PRODUITS DE CULTURE CELLULAIRE
Abstract:
(EN) An integrated cell culture-protein purification system has been developed for the continuous, automated production of pure cell culture protein products. The instrument comprises a bioreactor subunit (2) having a hollow fiber bioreactor to culture and maintain cells which secrete the desired product into the cell culture medium. The culture medium is circulated through a purification cartridge (4) which adsorbs the desired product. The system is capable of continuously removing the product from the culture medium by immunoaffinity adsorption and pure product is then recovered. The product can also be recovered automatically in a discontinuous operation from the spent culture medium by either affinity, ion exchange, or hydrophobic purification techniques. The integrated system allows continuous production of high-quality pure proteins from cell culture.
(FR) Un système intégré de culture de cellules-purification de protéines a été développé pour la production continue et automatisée de produits de protéine de culture de cellules pures. L'instrument comprend une sous-unité (2) d'un bioréacteur ayant un bioréacteur à fibre creuse pour cultiver et maintenir des cellules qui sécrètent le produit désiré dans le milieu de culture de cellules. Le milieu de culture circule au travers d'une cartouche (4) de purification qui adsorbe le produit désiré. Le système peut extraire en continu le produit du milieu de culture par adsorption par immunoaffinité, puis le produit pur est récupéré. Le produit peut également être récupéré automatiquement en discontinu du milieu de culture usé soit par affinité, soit par échange d'ion, soit par purification hydrophobe. Le système intégré permet la production en continu de protéines pures de grande qualité à partir d'une culture de cellules.
Designated States: AU, DK, FI, JP
European Patent Office (AT, BE, CH, DE, FR, GB, IT, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0455757AU1990063557