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1. WO1991000924 - METHOD FOR SEPARATING DNA FROM A SAMPLE AND KIT THEREFOR

Publication Number WO/1991/000924
Publication Date 24.01.1991
International Application No. PCT/US1990/003831
International Filing Date 09.07.1990
Chapter 2 Demand Filed 07.01.1991
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12N 15/1003
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Y10S 435/803
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
435Chemistry: molecular biology and microbiology
803Physical recovery methods, e.g. chromatography, grinding
Applicants
  • UNIVERSITY OF KANSAS [US/US]; Office of Research and Graduate Studies Rainbow Blvd., 39th Street Kansas City, KS 66103, US
Inventors
  • THOMAS, Stanley; US
  • TILZER, Lowell; US
  • MORENO, Ruben; US
Agents
  • HANSON, Norman, D.; Felfe & Lynch 805 Third Avenue New York, NY 10022, US
Priority Data
381,58810.07.1989US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR SEPARATING DNA FROM A SAMPLE AND KIT THEREFOR
(FR) METHODE DE SEPARATION D'ADN D'UN ECHANTILLON, ET KIT UTILISE DANS CETTE METHODE
Abstract
(EN)
The invention describes a method for extracting DNA from a sample. It involves contacting a sample with a separation reagent which permits differentiated solvation of DNA and protein. By adding a gel polymer, such as a silica gel polymer to the mixture of sample and separating reagent followed by agitation via, e.g., centrifugation, the DNA and protein are separated, with the gel acting as a block to prevent contamination of the DNA phase by the protein. Higher yields of DNA are obtained as compared to methodologies where the gel is not used. Additionally, the problems associated with the physical contact of the solvents, which are frequently carcinogens, are avoided. Also taught are kits which can be used in connection with the inventive method.
(FR)
L'invention concerne une méthode d'extraction d'ADN d'un échantillon. Elle consiste à mettre en contact un échantillon avec un réactif de séparation qui permet la solvation différentiée de l'ADN et de la protéine. En ajoutant un gel polymère tel qu'un gel polymère à base de silice au mélange d'échantillon et de réactif de séparation puis en effectuant une agitation par exemple par centrifugation, l'ADN et la protéine sont séparés et le gel joue le rôle de bloc empêchant la contamination de la phase d'ADN par la protéine. Des productions plus importantes d'ADN sont obtenues par rapport aux méthodes n'utilisant pas de gel. De plus, les problèmes associés au contact physique des solvants, qui sont fréquemment carcinogènes, sont éliminés. L'invention concerne également des kits qui peuvent être utilisés dans la méthode de l'invention.
Also published as
Latest bibliographic data on file with the International Bureau