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1. WO1991000905 - RAPID, VERSATILE AND SIMPLE SYSTEM FOR EXPRESSING GENES IN EUKARYOTIC CELLS

Publication Number WO/1991/000905
Publication Date 24.01.1991
International Application No. PCT/US1990/003687
International Filing Date 03.07.1990
Chapter 2 Demand Filed 03.07.1990
IPC
C12N 9/10 2006.01
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9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
C12N 9/12 2006.01
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9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
C12N 15/10 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/67 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
C12N 15/85 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C12N 15/863 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
863Poxviral vectors, e.g. vaccinia virus
CPC
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/67
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C12N 15/86
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2710/24143
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2710dsDNA Viruses
00011dsDNA Viruses
24011Poxviridae
24111Orthopoxvirus, e.g. vaccinia virus, variola
24141Use of virus, viral particle or viral elements as a vector
24143viral genome or elements thereof as genetic vector
C12N 2770/32243
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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2770ssRNA Viruses positive-sense
00011ssRNA Viruses positive-sense
32011Picornaviridae
32211Cardiovirus, e.g. encephalomyocarditis virus
32241Use of virus, viral particle or viral elements as a vector
32243viral genome or elements thereof as genetic vector
Applicants
  • THE UNITED STATES OF AMERICA, as represented by the Secretary, U.S. DEPARTMENT OF COMMERCE [US/US]; 5285 Port Royal Road Springfield, VA 22161, US
Inventors
  • MOSS, Bernard; US
  • FUERST, Thomas; US
  • ELROY-STEIN, Orna; US
Agents
  • HOLMAN, John, Clarke ; Fleit, Jacobson, Cohn, Price, Holman & Stern The Jenifer Building 400 Seventh Street, N.W. Washington, DC 20004, US
Priority Data
376,68707.07.1989US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) RAPID, VERSATILE AND SIMPLE SYSTEM FOR EXPRESSING GENES IN EUKARYOTIC CELLS
(FR) SYSTEME RAPIDE, VERSATILE ET SIMPLE D'EXPRESSION DE GENES DANS DES CELLULES EUKARYOTIQUES
Abstract
(EN)
An efficient, versatile and simple expression system which confers cap-independent translation to prokaryotic RNAs in eukaryotic cells has been described. The utility of recombinant vaccinia virus for this purpose has been illustrated.
(FR)
L'invention concerne un système d'expression efficace, versatile et simple qui confère une translation indépendante de l'enveloppe aux ARNs prokaryotiques dans des cellules eukaryotiques. L'utilité du virus de la vaccine de recombinaison à cet effet a été illustré.
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