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1. WO1991000762 - PERFUSIVE CHROMATOGRAPHY

Publication Number WO/1991/000762
Publication Date 24.01.1991
International Application No. PCT/US1990/000930
International Filing Date 20.02.1990
IPC
B01D 15/34 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08Selective adsorption, e.g. chromatography
26characterised by the separation mechanism
34Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
G01N 30/52 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
50Conditioning of the sorbent material or stationary liquid
52Physical parameters
CPC
B01D 15/345
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents
08Selective adsorption, e.g. chromatography
26characterised by the separation mechanism
34Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
345Perfusive chromatography
B01J 20/28095
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
28characterised by their form or physical properties
28054characterised by their surface properties or porosity
28095Shape or type of pores, voids, channels, ducts
B01J 20/282
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
281Sorbents specially adapted for preparative, analytical or investigative chromatography
282Porous sorbents
B01J 2220/54
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2220Aspects relating to sorbent materials
50Aspects relating to the use of sorbent or filter aid materials
54Sorbents specially adapted for analytical or investigative chromatography
G01N 2030/528
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
50Conditioning of the sorbent material or stationary liquid
52Physical parameters
524structural properties
528Monolithic sorbent material
Applicants
  • PERSEPTIVE BIOSYSTEMS, INC. [US/US]; 60 Hamilton Street Cambridge, MA 02139, US
Inventors
  • AFEYAN, Noubar, B.; US
  • REGNIER, Fred, E.; US
  • DEAN, Robert, C., Jr.; US
Agents
  • PITCHER, Edmund, R. ; Lahive & Cockfield 60 State Street Boston, MA 02109, US
Priority Data
376,88506.07.1989US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PERFUSIVE CHROMATOGRAPHY
(FR) CHROMATOGRAPHIE PAR PERFUSION
Abstract
(EN)
Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores (14). The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores (14) within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.
(FR)
On a mis au point des procédés de chromatographie ainsi que des géométries matricielles permettant la séparation à haute résolution et à productivité élevée de mélanges de solutés, notamment de matières biologiques. Le procédé consiste à passer des fluides dans des matrices de chromatographie spécialement conçues, à des débits élevés. Les matrices définissent des premier et second ensembles interconnectés de pores, ainsi qu'une grande surface permettant l'interaction de soluté, en communication fluide avec les éléments du second ensemble de pores (14). Les premier et second ensembles de pores sont matérialisés, par exemple, par les interstices entre des particules et des pores de passage (14) se trouvant à l'intérieur des particules. Les pores sont dimensionnés de sorte que, à des débits de fluide élevés pouvant être atteints, un écoulement par convexion a lieu dans les deux ensembles de pores, et le débit de convexion dépasse la vitesse de la diffusion de solutés dans le second ensemble de pores. Cette approche associe le transport de masse à convexion et à diffusion vers la surface active et inversement, et permet des augmentations de la vitesse du fluide sans l'étalage de la bande normalement attendu.
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