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1. WO1991000359 - PARTICLE-MEDIATED TRANSFORMATION OF ANIMAL SOMATIC CELLS

Publication Number WO/1991/000359
Publication Date 10.01.1991
International Application No. PCT/US1990/003522
International Filing Date 21.06.1990
IPC
C12M 3/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
C12N 15/89 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
89using microinjection
CPC
C12M 35/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
35Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
C12N 15/87
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
C12N 15/895
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
89using microinjection
895using biolistic methods
Applicants
  • AGRACETUS, INC. [US/US]; 8520 University Green Middleton, WI 53562, US
Inventors
  • BRILL, Winston, J.; US
  • McCABE, Dennis, E.; US
  • YANG, Ning-Sun; US
Agents
  • SEAY, Nicholas, J.; Quarles & Brady P.O. Box 2113 Madison, WI 53701-2113, US
Priority Data
371,86926.06.1989US
494,93314.03.1990US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PARTICLE-MEDIATED TRANSFORMATION OF ANIMAL SOMATIC CELLS
(FR) TRANSFORMATION AU MOYEN DE PARTICULES DE CELLULES SOMATIQUES ANIMALES
Abstract
(EN)
A method is disclosed for the convenient transformation of the somatic cells of animals. Somatic cell transformation is useful for medical and veterinary care of genetic diseases, and other therapeutic or animal improvement purposes. The method makes use of an electric discharge particle acceleration apparatus which can inject very small particles of gold or other dense material carrying genetic constructs coated on them into the living cells of animals. The animals not only live, but there is no visible bruising or bleeding at the site of the treatment. The method is particularly adaptable since the force of the particle injection in such a spark discharge apparatus is adjustable by adjustments to the voltage of the spark discharge.
(FR)
Méthode pour transformer commodément les cellules somatiques d'animaux. La transformation de celles somatiques s'utilise dans le traitement médical et vétérinaire de maladies génétiques, ainsi qu'à d'autres fins thérapeutiques ou en vue d'améliorer les races animales. Cette méthode utilise un appareil d'accélération des particules par décharge électrique, qui peut injecter dans les cellules animales vivantes de minuscules particules d'or ou d'autres matières denses porteuses de constructions génétiques. Les animaux sont non seulement vivants, mais ne portent aucun signe visible d'ecchymose ni de saignement à l'endroit du traitement. Cette méthode est particulièrement souple puisque la puissance d'injection des particules dans l'appareil est réglable par le réglage de la tension de la décharge d'étincelles.
Also published as
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