Processing

Please wait...

Settings

Settings

1. WO1991000353 - MUTANT MICROBIAL $g(a)-AMYLASES WITH INCREASED THERMAL, ACID AND/OR ALKALINE STABILITY

Publication Number WO/1991/000353
Publication Date 10.01.1991
International Application No. PCT/EP1990/001042
International Filing Date 27.06.1990
IPC
C12N 9/26 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
24acting on glycosyl compounds (3.2)
26acting on alpha-1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
C12N 9/28 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
24acting on glycosyl compounds (3.2)
26acting on alpha-1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
28Alpha-amylase from microbial source, e.g. bacterial amylase
C12N 15/70 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
C12N 15/75 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
75for Bacillus
CPC
C12N 15/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
C12N 15/75
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
75for Bacillus
C12N 9/2414
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
24acting on glycosyl compounds (3.2)
2402hydrolysing O- and S- glycosyl compounds (3.2.1)
2405Glucanases
2408acting on alpha -1,4-glucosidic bonds
2411Amylases
2414Alpha-amylase (3.2.1.1.)
C12N 9/2417
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
24acting on glycosyl compounds (3.2)
2402hydrolysing O- and S- glycosyl compounds (3.2.1)
2405Glucanases
2408acting on alpha -1,4-glucosidic bonds
2411Amylases
2414Alpha-amylase (3.2.1.1.)
2417from microbiological source
Applicants
  • GIST-BROCADES N.V. [NL/NL]; Wateringseweg 1 P.O. Box 1 NL-2600 MA Delft, NL (AllExceptUS)
  • PLANT GENETIC SYSTEMS N.V. [BE/BE]; Kolonel Bourgstraat 106 Bus 6 B-1040 Brussels, BE (AllExceptUS)
  • QUAX, Wilhelmus, Johannes [NL/NL]; NL (UsOnly)
  • LAROCHE, Yves [BE/BE]; BE (UsOnly)
  • VOLLEBREGT, Adrianus, Wilhelmus, Hermanus [NL/NL]; NL (UsOnly)
  • STANSSENS, Patrick [BE/BE]; BE (UsOnly)
  • LAUWEREYS, Marc [BE/BE]; BE (UsOnly)
Inventors
  • QUAX, Wilhelmus, Johannes; NL
  • LAROCHE, Yves; BE
  • VOLLEBREGT, Adrianus, Wilhelmus, Hermanus; NL
  • STANSSENS, Patrick; BE
  • LAUWEREYS, Marc; BE
Agents
  • HUYGENS, Arthur, Victor ; Gist-Brocades N.V. Patents and Trademarks Department Wateringseweg 1 P.O. Box 1 NL-2600 MA Delft, NL
Priority Data
89201735.129.06.1989EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MUTANT MICROBIAL $g(a)-AMYLASES WITH INCREASED THERMAL, ACID AND/OR ALKALINE STABILITY
(FR) $g(a)-AMYLASES MICROBIENNES MUTANTES PRESENTANT UNE MEILLEURE STABILITE THERMIQUE, AUX ACIDES ET/OU AUX ALCALINS
Abstract
(EN)
Thermostable and acid stable $g(a)-amylases are provided as expression products of genetically engineered $g(a)-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant $g(a)-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.
(FR)
L'invention concerne des $g(a)-amylases thermostables et stables à l'acide, à titre de produits d'expression de gènes d'$g(a)-amylases mise au point génétiquement, isolé à partir de microorganismes, appartenant de préférence à la classe des Bacilli. Les procédés de mutagénèse à la fois chimique et enzymatique sont, par exemple, le procédé au sulfite acide ainsi que l'incorporation érronée enzymatique dans de l'ADN hétéroduplex espacé. Les $g(a)-amylases mutantes présentent des propriétés supérieures, par exemple une thermostabilité améliorée sur une large plage de pH, destinées à des applications industrielles dans le traitement de l'amidon et dans le désencollage de textiles.
Latest bibliographic data on file with the International Bureau