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1. WO1991000288 - HYDROPHOBIC NUCLEIC ACID PROBE

Publication Number WO/1991/000288
Publication Date 10.01.1991
International Application No. PCT/US1990/003623
International Filing Date 27.06.1990
Chapter 2 Demand Filed 30.01.1991
IPC
C07H 21/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C07H 21/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12Q 1/6834
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
Applicants
  • CHIRON CORPORATION [US/US]; US
Inventors
  • CHIRON CORPORATION; US
  • URDEA, Michael, S.; US
  • HORN, Thomas; US
Agents
  • SHETKA, Debra, A. ; Irell & Manella 545 Middlefield Road Suite 200 Menlo Park, CA 94025, US
Priority Data
374,46230.06.1989US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) HYDROPHOBIC NUCLEIC ACID PROBE
(FR) SONDE A ACIDE NUCLEIQUE HYDROPHOBE
Abstract
(EN)
A process is disclosed for immobilizing a polynucleotide possessing a hydrophobic moiety on a terminal nucleotide onto a solid substrate that permits only end-point attachment, thereby allowing the polynucleotide to be fully available for hybridization. This method of polynucleotide attachment overcomes the prior art problem of multi-site and internal attached sites of polynucleotide to the substrate.
(FR)
Le procédé décrit sert à immobiliser sur un substrat solide un polynucléotide comportant sur un nucléotide terminal une fraction hydrophobe, de façon à permettre uniquement une fixation par points d'extrémité. Le polynucléotide est alors complètement disponible pour être soumis à hybridation. Ce procédé de fixation de polynucléotides élimine les désavantages de la technique actuelle, à savoir la fixation des polynucléotides sur le substrat par sites multiples ou par sites fixés intérieurement.
Also published as
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