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1. WO1991000047 - RETROVIRUS DETECTION

Publication Number WO/1991/000047
Publication Date 10.01.1991
International Application No. PCT/US1990/003717
International Filing Date 29.06.1990
Chapter 2 Demand Filed 23.01.1991
IPC
C12N 15/867 2006.01
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
867Retroviral vectors
C12Q 1/04 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C12Q 1/68 2006.01
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1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C12Q 1/70 2006.01
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1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
G01N 33/564 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
564for pre-existing immune complex or autoimmune disease
G01N 33/569 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
CPC
C12N 15/86
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2740/13043
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2740Reverse Transcribing RNA Viruses
00011Reverse Transcribing RNA Viruses
10011Retroviridae
13011Gammaretrovirus, e.g. murine leukeamia virus
13041Use of virus, viral particle or viral elements as a vector
13043viral genome or elements thereof as genetic vector
C12Q 1/04
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1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C12Q 1/6897
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QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6897involving reporter genes operably linked to promoters
C12Q 1/70
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QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
C12Q 1/702
CCHEMISTRY; METALLURGY
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QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
701Specific hybridization probes
702for retroviruses
Applicants
  • THE REGENTS OF THE UNIVERSITY OF CALIFORNIA [US/US]; 2199 Addison Street Berkeley, CA 94720, US
Inventors
  • GRUBER, Harry, E.; US
  • JOLLY, Douglas, J.; US
  • KWANG, Hwei-Sing; US
Agents
  • DEVEZA, Robert, R. ; Knobbe, Martens, Olson & Bear 620 Newport Center Drive Newport Beach, CA 92660, US
Priority Data
373,45830.06.1989US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) RETROVIRUS DETECTION
(FR) DETECTION DE RETROVIRUS
Abstract
(EN)
A method, and groups of cells and retroviruses useful in the performance of the method, for testing for the presence or amount of a first retrovirus in a specimen. A replication defective retroviral vector construct having a marker gene sequence is exposed to the specimen and the presence or amount of a recombinant or pseudotyped retrovirus carrying the marker gene sequence is tested for. The replication defective vector may be disposed in cells, with the specimen being tested for the presence or amount of a retrovirus, or it may be disposed in a retrovirus, with the specimen being a group of cells which are tested for the presence or amount of said first retrovirus. In either case, where a first retrovirus complements the defective retroviral vector construct, a recombinant or pseudotyped retrovirus carrying the marker gene sequence will be produced. The first retrovirus to be tested for may be of a previously known, or unknown type, and may be any of a group of retroviruses.
(FR)
Procédé, ainsi que groupes de cellules et de rétrovirus utiles dans la mise en ÷uvre du procédé, pour effectuer un test afin de déceler la présence ou une quantité d'un premier rétrovirus dans un spécimen. Une construction vectorielle rétrovirale défectueuse de réplication ayant une séquence génétique marqueuse est exposée au spécimen, et la présence ou une quantité d'un rétrovirus recombinant ou pseudotypé portant la séquence génétique marqueuse est détectée. Le vecteur défectueux de réplication peut être placé dans des cellules, le spécimen étant testé pour déceler la présence ou une quantité d'un rétrovirus, ou bien il peut être placé dans un rétrovirus, le spécimen étant un groupe cellulaire testé pour déceler la présence ou une quantité dudit premier rétrovirus. Dans les deux cas, là où un premier rétrovirus complète la construction vectorielle rétrovirale défectueuse, un rétrovirus recombinant ou pseudotypé portant la séquence génétique marqueuse sera produit. Le premier rétrovirus dont il faut déceler la présence peut être d'un type déjà connu ou inconnu, et peut être n'importe lequel d'un groupe de rétrovirus.
Also published as
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