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Machine translation
1. (WO1990015065) EXONUCLEASE-RESISTANT OLIGONUCLEOTIDES AND METHODS FOR PREPARING THE SAME
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1990/015065    International Application No.:    PCT/US1990/003138
Publication Date: 13.12.1990 International Filing Date: 05.06.1990
Chapter 2 Demand Filed:    03.01.1991    
IPC:
C07H 21/00 (2006.01)
Applicants: GILEAD SCIENCES, INC. [US/US]; 344 Lakeside Drive, Foster City, CA 94404 (US) (For All Designated States Except US).
FROEHLER, Brian, C. [US/US]; (US) (For US Only)
Inventors: FROEHLER, Brian, C.; (US)
Agent: REED, Dianne, E.; Irell & Manella, 545 Middlefield Road, Suite 200, Menlo Park, CA 94025 (US)
Priority Data:
361,045 05.06.1989 US
Title (EN) EXONUCLEASE-RESISTANT OLIGONUCLEOTIDES AND METHODS FOR PREPARING THE SAME
(FR) OLIGONUCLEOTIDES RESISTANT A L'EXONUCLEASE, ET PROCEDES POUR LEUR PREPARATION
Abstract: front page image
(EN)A method is provided for making 3' and/or 5' end-capped oligonucleotides so as to render the oligonucleotide resistant to degradation by exonucleases. The exonuclease degradation resistance is provided by incorporating two or more phosphoramidate and phosphoromonothioate and/or phosphorodithioate linkages at the 5' and/or 3' ends of the oligonucleotide, wherein the number of phosphoramidate linkages is less than a number which would interfere with hybridization to a complementary oligonucleotide strand and/or which would interfere with RNAseH activity when the oligonucleotide is hybridized to RNA.
(FR)On prévoit un procédé pour la préparation d'oligonicleotides ayant des terminaisons 3' et/ou 5' de manière à rendre l'oligonucléotide résistant à la décomposition provoquée par des exonucléasés. Ladite résistance est réalisée en incorporant deux ou plusieurs liaisons de phosphoramidate et phosphoromonothioate et/ou phosphorodithioate aux terminaisons 5' et/ou 3' de l'olignucléotide, où le nombre de liaisons de phosphoramidate est inférieur à un nombre pouvant perturber l'hybridation avec un brin d'oligonucléotide complémentaire et/ou pouvant perturber une activité ARNseH lorsque l'oligonucléotide est hybridé avec l'ARN.
Designated States: CA, JP, KR, US.
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, IT, LU, NL, SE).
Publication Language: English (EN)
Filing Language: English (EN)