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1. WO1990014097 - IMPROVED CULTURE OF HUMAN TUMOR-INFILTRATING LYMPHOCYTES BY COMBINING GAS PERMEABLE BAGS AND A PRIMARY CULTURE CONTAINER

Publication Number WO/1990/014097
Publication Date 29.11.1990
International Application No. PCT/US1990/002671
International Filing Date 11.05.1990
Chapter 2 Demand Filed 30.11.1990
IPC
A61K 35/14 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
14Blood; Artificial blood
A61K 38/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C12N 5/0783 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
078Cells from blood or from the immune system
0783T cells; NK cells; Progenitors of T or NK cells
CPC
A61K 2039/5158
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
51comprising whole cells, viruses or DNA/RNA
515Animal cells
5158Antigen-pulsed cells, e.g. T-cells
A61K 38/2013
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
17from animals; from humans
19Cytokines; Lymphokines; Interferons
20Interleukins [IL]
2013IL-2
A61K 39/0011
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
0005Vertebrate antigens
0011Cancer antigens
C12N 2500/90
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2500Specific components of cell culture medium
90Serum-free medium, which may still contain naturally-sourced components
C12N 2501/23
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
20Cytokines; Chemokines
23Interleukins [IL]
C12N 5/0636
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0634Cells from the blood or the immune system
0636T lymphocytes
Applicants
  • E.I. DU PONT DE NEMOURS AND COMPANY [US]/[US]
Inventors
  • NAHAPETIAN, Ara, Toonnaz
  • WIEDMEYER, Jerilou, Ann
Agents
  • HORN, Margaret, A.
Priority Data
351,92015.05.1989US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) IMPROVED CULTURE OF HUMAN TUMOR-INFILTRATING LYMPHOCYTES BY COMBINING GAS PERMEABLE BAGS AND A PRIMARY CULTURE CONTAINER
(FR) CULTURE AMELIOREE DE LYMPHOCYTES D'INFILTRATION DE TUMEURS PAR COMBINAISON DE SACS PERMEABLES AUX GAZ ET D'UN RECIPIENT DE CULTURE PRIMAIRE
Abstract
(EN)
There is disclosed a process for the enhanced expansion and maintenance of human tumor-infiltrating lymphocytes (TIL). The process comprises placing a human tumor sample in a primary culture container and maintaining the sample in the primary culture container for a period of time before transferring a substantial amount of the primary culture to a bulk culture container. The primary culture cells and/or conditioned medium retained in the primary culture container are periodically used to supplement the bulk culture. Both primary and bulk cultures are maintained and expanded by periodic addition of a suitable culture medium as needed.
(FR)
L'invention concerne un procédé de développement et d'entretien améliorés de lymphocytes d'infiltration de tumeurs (LIT). Ledit procédé consiste à placer un échantillon de tumeur humaine dans un récipient de culture primaire, et à entretenir ledit échantillon dans ledit récipient de culture primaire pendant une période de temps donnée avant de transférer une quantité substantielle de ladite culture primaire dans un récipient de culture volumique. Les cellules de culture primaire et/ou le milieu conditionné maintenus dans le récipient de culture primaire sont utilisés périodiquement afin de compléter la culture volumique. Les cultures à la fois primaire et volumique sont entretenues et développées par l'addition périodique d'un milieu de culture adapté, en fonction des besoins.
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