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1. WO1990012889 - METHOD OF ANALYSIS, REAGENT COMPOSITION AND USE THEREOF FOR GLUCOSE DETERMINATION

Publication Number WO/1990/012889
Publication Date 01.11.1990
International Application No. PCT/SE1990/000272
International Filing Date 24.04.1990
Chapter 2 Demand Filed 15.11.1990
IPC
C12Q 1/54 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
54involving glucose or galactose
CPC
C12Q 1/54
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
54involving glucose or galactose
Applicants
  • MIGRATA UK LTD [GB]/[GB] (AllExceptUS)
  • LILJA, Jan, Evert [SE]/[SE] (UsOnly)
  • NILSSON, Sven-Erik, Lennart [SE]/[SE] (UsOnly)
Inventors
  • LILJA, Jan, Evert
  • NILSSON, Sven-Erik, Lennart
Agents
  • AWAPATENT AB
Priority Data
8901515-025.04.1989SE
Publication Language English (EN)
Filing Language Swedish (SV)
Designated States
Title
(EN) METHOD OF ANALYSIS, REAGENT COMPOSITION AND USE THEREOF FOR GLUCOSE DETERMINATION
(FR) PROCEDE D'ANALYSE, COMPOSITION D'AGENT REACTIF ET UTILISATION DE CELLE-CI POUR LA DETERMINATION DU GLUCOSE
Abstract
(EN)
A method, a reagent composition and the use thereof for quantitative determination of total glucose in undiluted whole blood are described. A sample of undiluted whole blood is contacted with a reagent composition which is in dry form and comprises: glucose dehydrogenase (GDH); one or more substances from the group consisting of diaphorase, phenazine methosulphate, phenazine ethosulphate, phenazine phenosulphate and Meldola blue; one or more substances from the group consisting of NAD, NADP, thio-NAD, thio-NADP, nicotinamide-purine dinucleotide, nicotinamide-methylpurine dinucleotide, nicotinamide-2-chloro-methylpurine dinucleotide; one or more hemolysing substances from the group consisting of phospholipase, hemolysing saponins, and compounds of hydrophilic mono-, di- or tri-saccharides and aliphatic hydrocarbons having 10-16 carbon atoms; a redox indicator dye; and optionally mutarotase. A colour change brought about by the reaction of the reagent with glucose in the undiluted whole blood is measured by transmission spectrophotometry.
(FR)
Un procédé, une composition d'agent réactif, et l'utilisation de celle-ci pour la détermination quantitative du glucose total dans du sang entier non dilué sont décrits. Un échantillon de sang entier non dilué est mis en contact avec une composition d'agent réactif sous forme sèche comprenant de la glucose-déshydrogénase (GDH); une ou plusieurs substances du groupe qui consiste de: diaphorase, méthosulfate de phénazine, éthosulfate de phénazine, phénosulfate de phénazine et Meldola blue; une ou plusieurs substances du groupe qui consiste de: NAD, NADP, thio-NAD, thio-NADP nicotinamide-purine-dinucléotide, nicotinamide-méthylpurine-dinucléotide, nicotinamide-2-chloro-méthylpurine-dinucléotide; une ou plusieurs substances hémolysantes du groupe qui consiste de: phospholipase, des saponines hémolysantes et des composés de mono-, di- ou tri-saccharides hydrophiles et des hydrocarbures aliphatiques ayant 10-16 atomes de carbone; un colorant indicateur redox et, de façon facultative, de la mutata rose. Une modification de couleur, effectuée au cours d'une réaction de l'agent réactif avec du glucose dans le sang entier non dilué, est mesurée par spectrophotométrie de transmission.
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