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1. (WO1989012679) METHOD FOR PRODUCING THERMALLY STABLE ALPHA-AMYLASE
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/1989/012679 International Application No.: PCT/US1989/002683
Publication Date: 28.12.1989 International Filing Date: 19.06.1989
IPC:
C12N 9/28 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
24
acting on glycosyl compounds (3.2)
26
acting on alpha-1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
28
Alpha-amylase from microbial source, e.g. bacterial amylase
Applicants:
GREAT LAKES CHEMICAL CORPORATION [US/US]; P.O. Box 2200 Highway 52, N.W. West Lafayette, IN 47906, US
Inventors:
CHEN, James, C.; US
LAMM, William, R.; US
RACINE, Francis, M.; US
TURNER, Hal, R.; US
Agent:
VITTUM, Daniel, W., Jr. @; Kirkland & Ellis 200 East Randolph Drive Suite 6100 Chicago, IL 60601, US
Priority Data:
209,42621.06.1988US
Title (EN) METHOD FOR PRODUCING THERMALLY STABLE ALPHA-AMYLASE
(FR) PROCEDE DE PRODUCTION D'ALPHA-AMYLASE THERMIQUEMENT STABLE
Abstract:
(EN) A process for the production of thermally stable $i(alpha-amylase) comprising the steps of: cultivating $i(B. licheniformis) ATCC 53,722 in a nutrient medium which contains as a primary carbon source a carbohydrate which normally represses alpha-amylase synthesis, selected from the group consisting of dextrose, starch hydrolysate, glycerol, xylose, mannose, sucrose and mixtures thereof; feeding said primary carbon source to said medium during cultivation; maintaining the level of carbon dioxide evolution from the medium during cultivation between about 4.5 % and 6.5 % of the off-gas when the air flow is 0.5 to 1.0 vvm by controlling the rate at which the primary carbon source is fed to said medium during cultivation; and maintaining the pH of the medium between 6.5 and 7.0.
(FR) Un procédé de production d'$i(alpha-amylase) thermiquement stable comprend les étapes suivantes: la culture de $i(B. licheniformis), ATCC 53.722, dans un milieu nutritif qui contient comme source primaire de carbone un hydrate de carbone qui normalement inhibe la synthèse d'alpha-amylase, sélectionné dans le groupe formé par dextrose, hydrolysat d'amidon, glycérol, xylose, sucrose et leurs mélanges; l'adjonction de cette source primaire de carbone au milieu nutritif pendant la culture; le maintien du niveau de dioxyde de carbone dégagé par le milieu pendant la culture entre 4,5 % et 6,5 % des effluents gazeux lorsque le débit d'air est compris entre 0,5 et 1,0 vvm en ajustant le taux d'adjonction de la source primaire de carbone au milieu pendant la culture; et le maintien du pH du milieu nutritif entre 6,5 et 7,0.
Designated States: BG, BR, DK, FI, HU, JP, KR, SU
European Patent Office (EPO) (AT, BE, CH, DE, FR, GB, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)