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1. (WO1989006279) MUTATED SUBTILISIN GENES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1989/006279 International Application No.: PCT/DK1989/000002
Publication Date: 13.07.1989 International Filing Date: 06.01.1989
Chapter 2 Demand Filed: 09.08.1989
IPC:
C11D 3/386 (2006.01) ,C12N 9/54 (2006.01)
C CHEMISTRY; METALLURGY
11
ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
D
DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
3
Other compounding ingredients of detergent compositions covered in group C11D1/101
16
Organic compounds
38
Products with no well-defined composition
386
Preparations containing enzymes
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
48
acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50
Proteinases
52
derived from bacteria
54
bacteria being Bacillus
Applicants:
NOVO-NORDISK A/S [DK/DK]; Novo Allé DK-2880 Bagsværd, DK
Inventors:
HASTRUP, Sven; DK
BRANNER, Sven; DK
NORRIS, Fanny; DK
PETERSEN, Steffen, B.; DK
NØRSKOV-LAURIDSEN, Leif; DK
JENSEN, Villy, Johannes; DK
AASLYNG, Dorrit; DK
Priority Data:
64/8807.01.1988DK
Title (EN) MUTATED SUBTILISIN GENES
(FR) GENES DE SUBTILISINE MUTES
Abstract:
(EN) The present invention relates to mutations of the subtilisin gene, some of which result in changes in the chemical characteristics of subtilisin enzyme. Mutations are created at specific nucleic acids of the subtilisin gene and, in various specific embodiments, the mutant enzymes possess altered chemical properties including, but not limited to, increased stability to oxidation, augmented proteolytic activity, and improved washability. The present invention also relates, but is not limited to, the amino acid and DNA sequences for two proteases derived from $i(Bacillus lentus) variants, subtilisin 147 and subtilisin 309, as well as mutations of these genes and the corresponding mutant enzymes.
(FR) La présente invention concerne des mutations de gène de subtilisine, dont certaines ont pour résultat des modifications des caractéristiques chimiques de l'enzyme de subtilisine. On crée des mutations pour des acides nucléiques spécifiques du gène de subtilisine et, dans divers modes de réalisation spécifiques, les enzymes mutantes possèdent des propriétés chimiques modifiées comprenant entre autres une stabilité accrue à l'oxydation, une activité protéolytique augmentée et une aptitude au lavage améliorée. La présente invention concerne également entre autres choses les séquences d'aminoacides et d'ADN pour deux protéases dérivées de variantes de $i(Bacillus lentus), la subtilisine 147 et la subtilisine 309, ainsi que des mutations de ces gènes et les enzymes mutantes correspondantes.
Designated States: DK, JP
European Patent Office (AT, BE, CH, DE, FR, GB, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0396608EP0675196EP1498481EP1538204US5741694US6506589
US20030148495US20030175933US20030186378US20050003986JP6292577 JP10113179
JPH03503477DE000068926163DKPR 175697DKPR 176102DK6488DK176102
DK175697