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1. (WO1989000605) TRANSFECTED CELLS CONTAINING PLASMIDS HAVING GENES ORIENTED IN OPPOSING DIRECTIONS AND METHODS OF OBTAINING THE SAME
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1989/000605 International Application No.: PCT/US1988/002411
Publication Date: 26.01.1989 International Filing Date: 15.07.1988
Chapter 2 Demand Filed: 21.02.1989
IPC:
C12N 15/85 (2006.01) ,C12N 9/72 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
48
acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
72
Urokinase
Applicants:
CODON [US/US]; 213 East Grand Avenue South San Francisco, CA 94070, US
Inventors:
CASHION, Linda, M.; US
BEGLEY, Kathi, A.; US
COLBY, Wendy, W.; US
MORSER, Michael, John; US
Agent:
WEBER, Kenneth, A.; Townsend and Townsend One Market Plaza 2000 Steuart Tower San Francisco, CA 94105, US
Priority Data:
074,08316.07.1987US
Title (EN) TRANSFECTED CELLS CONTAINING PLASMIDS HAVING GENES ORIENTED IN OPPOSING DIRECTIONS AND METHODS OF OBTAINING THE SAME
(FR) CELLULES TRANSFECTEES CONTENANT DES PLASMIDES COMPORTANT DES GENES ORIENTES DANS DES DIRECTIONS OPPOSEES ET LEUR PROCEDE D'OBTENTION
Abstract:
(EN) This invention relates generally to recombinant DNA techniques and to the expression of mammalian polypeptides in genetically engineered eukaryotic cells. Specifically, the invention relates to preferred plasmid constructs and methods that increase levels of expression of a cloned gene product. These preferred plasmids have selectable and nonselected gene cassettes adjacent to each other and oriented in the opposite direction for transcription. Such an orientation enhances overall levels of expression for the nonselected gene. Further, this invention relates to gene products expressed at these very high levels by the herein described method, and to the eukaryotic cells derived thereby.
(FR) Cette invention concerne des techniques recombinantes à base d'ADN ainsi que l'expression de poleptides mammifères dans des cellules eucaryotiques obtenues par génie génétique, plus précisément, elle se rapporte à des constructions de plasmides préférées et à des procédés améliorant les niveaux d'expression d'un produit génétique cloné. Ces plasmides préférés ont des cassettes génétiques sélectionnables et non sélectionnées, adjacentes les unes par rapport aux autres, et orientées dans la direction opposée pour transcription. Une telle orientation améliore les niveaux globaux d'expression pour le gène non sélectionné. En outre, cette invention a trait à des produits génétiques exprimés à ces niveaux très élevés par le procédé ici décrit, et à des cellules eucaryotiques ainsi dérivées.
Designated States: AU, JP
European Patent Office (AT, BE, CH, DE, FR, GB, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0368926AU1988022632