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1. WO1989000605 - TRANSFECTED CELLS CONTAINING PLASMIDS HAVING GENES ORIENTED IN OPPOSING DIRECTIONS AND METHODS OF OBTAINING THE SAME

Publication Number WO/1989/000605
Publication Date 26.01.1989
International Application No. PCT/US1988/002411
International Filing Date 15.07.1988
Chapter 2 Demand Filed 21.02.1989
IPC
C12N 15/85 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C12N 9/72 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
48acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
72Urokinase
CPC
C12N 15/85
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C12N 9/6459
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
48acting on peptide bonds (3.4)
50Proteinases ; , e.g. Endopeptidases (3.4.21-3.4.25)
64derived from animal tissue
6421from mammals
6424Serine endopeptidases (3.4.21)
6456Plasminogen activators
6459t-plasminogen activator (3.4.21.68), i.e. tPA
C12Y 304/21069
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
304Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
21Serine endopeptidases (3.4.21)
21069Protein C activated (3.4.21.69)
Applicants
  • CODON [US]/[US]
Inventors
  • CASHION, Linda, M.
  • BEGLEY, Kathi, A.
  • COLBY, Wendy, W.
  • MORSER, Michael, John
Agents
  • WEBER, Kenneth, A.
Priority Data
074,08316.07.1987US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) TRANSFECTED CELLS CONTAINING PLASMIDS HAVING GENES ORIENTED IN OPPOSING DIRECTIONS AND METHODS OF OBTAINING THE SAME
(FR) CELLULES TRANSFECTEES CONTENANT DES PLASMIDES COMPORTANT DES GENES ORIENTES DANS DES DIRECTIONS OPPOSEES ET LEUR PROCEDE D'OBTENTION
Abstract
(EN) This invention relates generally to recombinant DNA techniques and to the expression of mammalian polypeptides in genetically engineered eukaryotic cells. Specifically, the invention relates to preferred plasmid constructs and methods that increase levels of expression of a cloned gene product. These preferred plasmids have selectable and nonselected gene cassettes adjacent to each other and oriented in the opposite direction for transcription. Such an orientation enhances overall levels of expression for the nonselected gene. Further, this invention relates to gene products expressed at these very high levels by the herein described method, and to the eukaryotic cells derived thereby.
(FR) Cette invention concerne des techniques recombinantes à base d'ADN ainsi que l'expression de poleptides mammifères dans des cellules eucaryotiques obtenues par génie génétique, plus précisément, elle se rapporte à des constructions de plasmides préférées et à des procédés améliorant les niveaux d'expression d'un produit génétique cloné. Ces plasmides préférés ont des cassettes génétiques sélectionnables et non sélectionnées, adjacentes les unes par rapport aux autres, et orientées dans la direction opposée pour transcription. Une telle orientation améliore les niveaux globaux d'expression pour le gène non sélectionné. En outre, cette invention a trait à des produits génétiques exprimés à ces niveaux très élevés par le procédé ici décrit, et à des cellules eucaryotiques ainsi dérivées.
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