Search International and National Patent Collections
Some content of this application is unavailable at the moment.
If this situation persists, please contact us atFeedback&Contact
1. (WO1989000604) METHOD FOR IMPROVING TRANSLATION EFFICIENCY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1989/000604 International Application No.: PCT/US1988/002341
Publication Date: 26.01.1989 International Filing Date: 12.07.1988
IPC:
C07K 14/56 (2006.01) ,C12N 15/67 (2006.01) ,C12N 15/70 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
52
Cytokines; Lymphokines; Interferons
555
Interferons (IFN)
56
IFN-alpha
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67
General methods for enhancing the expression
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70
Vectors or expression systems specially adapted for E. coli
Applicants:
INTERFERON SCIENCES, INC. [US/US]; 783 Jersey Avenue New Brunswick, NJ 08901, US
Inventors:
LEE, Nancy; US
TESTA, Douglas; US
Agent:
FRANGOS, Irene, J.; National Patent Development Corporation 9 West 57th Street - Room 4170 New York, NY 10019, US
Priority Data:
072,72713.07.1987US
Title (EN) METHOD FOR IMPROVING TRANSLATION EFFICIENCY
(FR) PROCEDE PERMETTANT D'AMELIORER L'EFFICACITE DE TRANSLATION
Abstract:
(EN) A method for increasing the translation efficiency of a mRNA sequence is provided. In certain of its preferred embodiments, the method comprises the steps of: (a) constructing a predicted secondary structure for the mRNA; (b) analyzing the predicted secondary structure to determine if either or both of the AUG initiation codon and the Shine-Dalgarno sequence is contained in a double stranded portion of a stem-loop region of the predicted secondary structure; (c) calculating a free energy value for the stem-loop region; and (d) if the calculated free energy value is in the range of from zero to about -10.0 kcal/mole, modifying the DNA sequence for the mRNA so that when the modified sequence is transcribed it produces a modified mRNA sequence which has a predicted secondary structure wherein the AUG initiation codon and the Shine-Dalgarno sequence are not included in a double stranded portion of a stem-loop region of the predicted secondary structure. By means of this method, ten-fold increases in protein production have been achieved.
(FR) Le procédé décrit sert à améliorer l'efficacité de translation d'une séquence d'ARN messager (ARNm). Dans l'un de ses modes de réalisation préférés, ledit procédé consiste: (a) à construire une structure secondaire prédite pour l'ARNm; (b) à analyser la structure secondaire prédite pour déterminer si le codon d'initiation AUG et/ou la séquence Shine-Dalgarno sont contenus dans une partie torsadée double d'une région à tige en boucle de la structure secondaire prédite; (c) à calculer une valeur d'énergie libre pour la région à tige en boucle et (d), si la valeur d'énergie libre ainsi calculée est comprise entre 0 et environ -10,0 kcal/mole, à modifier la séquence d'ADN pour l'ARNm, de sorte que, lors de la transcription de la séquence modifiée, celle-ci produit une séquence d'ARNm modifiée qui comporte une structure secondaire prédite dans laquelle le codon d'initiation AUG et la séquence Shine-Dalgarno ne sont pas inclus dans une partie torsadée double d'une région à tige en boucle de la structure secondaire prédite. Grâce à ce procédé, on peut décupler la production de protéines.
Designated States: JP
European Patent Office (AT, BE, CH, DE, FR, GB, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0328584