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1. (WO1989000602) PROCESS FOR GENE TRANSFER IN PLANTS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1989/000602 International Application No.: PCT/EP1988/000635
Publication Date: 26.01.1989 International Filing Date: 13.07.1988
Chapter 2 Demand Filed: 10.02.1989
IPC:
A01H 4/00 (2006.01) ,C12N 15/82 (2006.01) ,C12N 5/04 (2006.01)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
4
Plant reproduction by tissue culture techniques
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
04
Plant cells or tissues
Applicants:
CHEMIE HOLDING AKTIENGESELLSCHAFT [AT/AT]; St.-Peter-Straße 25 A-4021 Linz, AT (AllExceptUS)
HEBERLE-BORS, Erwin [DE/AT]; AT (UsOnly)
BENITO MORENO, Rosa, Maria [ES/AT]; AT (UsOnly)
ALWEN, Anna [GB/AT]; AT (UsOnly)
Inventors:
HEBERLE-BORS, Erwin; AT
BENITO MORENO, Rosa, Maria; AT
ALWEN, Anna; AT
Agent:
KUNZ, Ekkehard; Chemie Holding AG Patentwesen St.-Peter-Straße 25 A-4021 Linz, AT
Priority Data:
P 37 24 154.021.07.1987DE
Title (EN) PROCESS FOR GENE TRANSFER IN PLANTS
(FR) PROCEDE DE TRANSFERT GENETIQUE ENTRE DES VEGETAUX
Abstract:
(EN) In a process for gene transfer in plants, a) unripe pollen grains are removed from the stamens, placed in nutrient solution, and the surrounding tissue is removed, b) the isolated unripe pollen grains are cultured in nutrient solution, c) exogenous genetic material is transferred to the pollen grains during the $i(in vitro) culturing and ripening, d) the transformed pollen grains are fully ripened $i(in vitro), e) the receiver plants are pollenated with the transformed pollen grains and their seeds are collected. The invention also concerns transgenic seeds and reproduction products.
(FR) Selon un procédé de transfert de gènes entre des végétaux a) on prélève des grains de pollen immatures dans des étamines, on les place dans une solution de culture et on enlève le tissu qui les entoure, b) on cultive les grains de pollen immatures ainsi isolés dans une solution de culture, c) on introduit du matériau génétique étranger dans les grains de pollen pendant leur culture et maturation $i(in vitro), d) on féconde des plantes réceptrices avec les grains de pollen ainsi transformés et on recueille les graines de celles-ci. L'invention concerne également des graines transgéniques et des produits de reproduction.
Designated States: AU, BB, BG, BR, DK, FI, HU, JP, KP, KR, LK, MC, MG, MW, NO, RO, SD, SU, US
European Patent Office (AT, BE, CH, DE, FR, GB, IT, LU, NL, SE)
African Intellectual Property Organization (BJ, CF, CG, CM, GA, ML, MR, SN, TD, TG)
Publication Language: German (DE)
Filing Language: German (DE)
Also published as:
ES2041286AR245216EP0301316EP0362293US5840557DE000003823712
AU1988020737