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1. (WO1989000600) STABLE, SINGLE-LIQUID ALPHA-AMYLASE REAGENT
Latest bibliographic data on file with the International Bureau
Pub. No.: WO/1989/000600 International Application No.: PCT/US1988/002414
Publication Date: 26.01.1989 International Filing Date: 15.07.1988
Chapter 2 Demand Filed: 24.04.1989
IPC:
C12Q 1/40 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
34
involving hydrolase
40
involving amylase
Applicants:
MODROVICH, Ivan, Endre [US/US]; US
Inventors:
MODROVICH, Ivan, Endre; US
KWAN, Shing, Sai; US
WORTHAM, Leon, Charles; US
Agent:
DILLARD, David, A.; Christie, Parker & Hale Post Office Box 7068 Pasadena, CA 91109-7068, US
Priority Data:
074,56917.07.1987US
Title (EN) STABLE, SINGLE-LIQUID ALPHA-AMYLASE REAGENT
(FR) REACTIF LIQUIDE STABLE MONOCONSTITUANT DE DOSAGE DE L'ALPHA-AMYLASE
Abstract:
(EN) A stable, single liquid alpha-amylase assay reagent comprises a polysaccharide or long chain oligosaccharide substrate for alpha-amylase having an optically detectable label bonded to the reducing end glucose, by a bond cleavable by alpha- or beta-glucosidase, and a blocking group bonded to the terminal end glucose. The reagent further comprises beta-amylase and either alpha- or beta-glucosidase. Sorbitol is provided in an amount of about 5 % to retard degradation of the enzymes. Zwitterionic buffers are also provided to stabilize the enzymes. In preparing the reagent, the enzymes and substrate are filtered through a 0.2 micron or less filter to remove alpha-amylase producing organisms.
(FR) Réactif liquide stable monoconstituant de dosage de l'alpha-amylase, comprenant un substrat de polysaccharide ou d'oligosaccharide à chaîne longue destiné à l'alpha-amylase et comportant un marqueur détectable optiquement, lié au glucose à extrémité réductrice par une liaison divisible par glucosidase alpha ou bêta, ainsi qu'un groupe de bloquage lié au glucose à extrémité terminale. Le réactif comprend en outre de la bêta-amylase et de la glucosidase soit alpha soit bêta. Du sorbitol dans une quantité d'environ 5 % retarde la dégradation des enzymes. Des tampons zwitterioniques stabilisent les enzymes. Lors de la préparation du réactif, on filtre les enzymes et le substrat à travers un filtre de 0,2 micron ou moins, afin de retirer les organismes produisant de l'alpha-amylase.
Designated States: JP
European Patent Office (AT, BE, CH, DE, FR, GB, IT, LU, NL, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0371994ES2009975JP2542251DE000003854127