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1. WO1989000600 - STABLE, SINGLE-LIQUID ALPHA-AMYLASE REAGENT

Publication Number WO/1989/000600
Publication Date 26.01.1989
International Application No. PCT/US1988/002414
International Filing Date 15.07.1988
Chapter 2 Demand Filed 24.04.1989
IPC
C12Q 1/40 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
34involving hydrolase
40involving amylase
CPC
C12Q 1/40
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
40involving amylase
C12Q 2334/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2334O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
10p-Nitrophenol derivatives
Applicants
  • MODROVICH, Ivan, Endre [US]/[US]
Inventors
  • MODROVICH, Ivan, Endre
  • KWAN, Shing, Sai
  • WORTHAM, Leon, Charles
Agents
  • DILLARD, David, A.
Priority Data
074,56917.07.1987US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) STABLE, SINGLE-LIQUID ALPHA-AMYLASE REAGENT
(FR) REACTIF LIQUIDE STABLE MONOCONSTITUANT DE DOSAGE DE L'ALPHA-AMYLASE
Abstract
(EN) A stable, single liquid alpha-amylase assay reagent comprises a polysaccharide or long chain oligosaccharide substrate for alpha-amylase having an optically detectable label bonded to the reducing end glucose, by a bond cleavable by alpha- or beta-glucosidase, and a blocking group bonded to the terminal end glucose. The reagent further comprises beta-amylase and either alpha- or beta-glucosidase. Sorbitol is provided in an amount of about 5 % to retard degradation of the enzymes. Zwitterionic buffers are also provided to stabilize the enzymes. In preparing the reagent, the enzymes and substrate are filtered through a 0.2 micron or less filter to remove alpha-amylase producing organisms.
(FR) Réactif liquide stable monoconstituant de dosage de l'alpha-amylase, comprenant un substrat de polysaccharide ou d'oligosaccharide à chaîne longue destiné à l'alpha-amylase et comportant un marqueur détectable optiquement, lié au glucose à extrémité réductrice par une liaison divisible par glucosidase alpha ou bêta, ainsi qu'un groupe de bloquage lié au glucose à extrémité terminale. Le réactif comprend en outre de la bêta-amylase et de la glucosidase soit alpha soit bêta. Du sorbitol dans une quantité d'environ 5 % retarde la dégradation des enzymes. Des tampons zwitterioniques stabilisent les enzymes. Lors de la préparation du réactif, on filtre les enzymes et le substrat à travers un filtre de 0,2 micron ou moins, afin de retirer les organismes produisant de l'alpha-amylase.
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